ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2013
Inducible presynaptic glutamine transport supports glutamatergic transmission at the calyx of Held synapse.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 42 citations in OpenAlex.
- PPT1 selectively depalmitoylates GAP43 to regulate neuronal excitability and cognitive function.Science advances · 2026Article
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- Amino Acid Transporters Proteins Involved in the Glutamate-Glutamine Cycle and Their Alterations in Murine Models of Alzheimer's Disease.Molecular neurobiology · 2024Review
- Aryl Hydrocarbon Receptor in Glia Cells: A Plausible Glutamatergic Neurotransmission Orchestrator.Neurotoxicity research · 2023Review
- Physiological synaptic activity and recognition memory require astroglial glutamine.Nature communications · 2022Article
- The Regulation of Astrocytic Glutamate Transporters in Health and Neurodegenerative Diseases.International journal of molecular sciences · 2020Review
- Fluoride Exposure Affects Glutamine Uptake in Müller Glia Cells.Neurotoxicity research · 2020Article
- D-Serine Signaling and NMDAR-Mediated Synaptic Plasticity Are Regulated by System A-Type of Glutamine/D-Serine Dual Transporters.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- GLAST Activity is Modified by Acute Manganese Exposure in Bergmann Glial Cells.Neurochemical research · 2020Article
- PKC-Mediated Modulation of Astrocyte SNAT3 Glutamine Transporter Function at Synapses in Situ.International journal of molecular sciences · 2018Article
- The role of glutamate transporters in the pathophysiology of neuropsychiatric disorders.NPJ schizophrenia · 2017Review
- Article
- Functional identification of activity-regulated, high-affinity glutamine transport in hippocampal neurons inhibited by riluzole.Journal of neurochemistry · 2017Article
- Mechanisms of Excessive Extracellular Glutamate Accumulation in Temporal Lobe Epilepsy.Neurochemical research · 2017Review
- Comparison of Glutamate Turnover in Nerve Terminals and Brain Tissue During [1,6-Neurochemical research · 2017Article
- The impact of glutamine supplementation on the symptoms of ataxia-telangiectasia: a preclinical assessment.Molecular neurodegeneration · 2016Article
- The homeostatic astroglia emerges from evolutionary specialization of neural cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- GLAST But Not Least--Distribution, Function, Genetics and Epigenetics of L-Glutamate Transport in Brain--Focus on GLAST/EAAT1.Neurochemical research · 2015Review
- Astroglial cradle in the life of the synapse.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Astroglial connexin 43 sustains glutamatergic synaptic efficacy.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms by which the excitatory neurotransmitter glutamate is recycled at synapses are currently unknown. By examining the functional expression of plasma membrane transporters at presynaptic terminals, we aim to elucidate some of the mechanisms of glutamate recycling. Using whole-cell voltage-clamp recordings from rat calyx of Held presynaptic terminals, our data show, for the first time, that the glutamate precursor glutamine causes the direct activation of an electrogenic, sodium-dependent presynaptic transporter, which supplies glutamine for generation of presynaptic glutamate and helps sustain synaptic transmission. Interestingly, the functional expression of this transporter at the presynaptic plasma membrane is dynamically controlled by electrical activity of the terminal, indicating that uptake of neurotransmitter precursors is controlled by the demand at an individual terminal. Induction of the transporter current is calcium-dependent and inhibited by botulinum neurotoxin C, demonstrating the involvement of SNARE-dependent exocytosis in inserting transporters into the plasma membrane when the terminal is active. Conversely, inactivity of the presynaptic terminal results in removal of transporters via clathrin-mediated endocytosis. To investigate whether the presynaptic glutamine transporter supplies the precursor for generating the synaptically released glutamate, we measured miniature EPSCs to assess vesicular glutamate content. When the presynaptic glutamate pool was turned over by synaptic activity, inhibiting the presynaptic glutamine transporters with MeAIB reduced the miniature EPSC amplitude significantly. This demonstrates that presynaptic glutamine transport is centrally involved in the production of glutamate and assists in maintaining excitatory neurotransmission.
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