ArticleJournal of immunology (Baltimore, Md. : 1950)2013
Substrate-induced protein stabilization reveals a predominant contribution from mature proteins to peptides presented on MHC class I.
Article in Journal of immunology (Baltimore, Md. : 1950), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- A poxvirus model reveals general correlates of antigen presentation and immunogenicity for viral CD8Science advances · 2025Article
- Kinetically distinct processing pathways diversify the CD8Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- When few survive to tell the tale: thymus and gonad as auditioning organs: historical overview.Theory in biosciences = Theorie in den Biowissenschaften · 2020Review
- Role for ribosome-associated quality control in sampling proteins for MHC class I-mediated antigen presentation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Designs of Antigen Structure and Composition for Improved Protein-Based Vaccine Efficacy.Frontiers in immunology · 2020Review
- Alternative Start Sites Downstream of Non-Sense Mutations Drive Antigen Presentation and Tolerance Induction to C-Terminal Epitopes.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Acute Pharmacologic Degradation of a Stable Antigen Enhances Its Direct Presentation on MHC Class I Molecules.Frontiers in immunology · 2017Article
- Remote and reversible inhibition of neurons and circuits by small molecule induced potassium channel stabilization.Scientific reports · 2016Article
- Connexins and pannexins in the immune system and lymphatic organs.Cellular and molecular life sciences : CMLS · 2015Review
- Lymphocyte repertoire selection and intracellular self/non-self-discrimination: historical overview.Immunology and cell biology · 2015Review
- The nature and extent of contributions by defective ribosome products to the HLA peptidome.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Re-examining class-I presentation and the DRiP hypothesis.Trends in immunology · 2014Review
- Monitoring peptide processing for MHC class I molecules in the endoplasmic reticulum.Current opinion in immunology · 2014Review
- Understanding the biology of antigen cross-presentation for the design of vaccines against cancer.Frontiers in immunology · 2014Review
- Effects of messenger RNA structure and other translational control mechanisms on major histocompatibility complex-I mediated antigen presentation.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The origin of the MHC class I-presented peptides are thought to be primarily from newly synthesized but defective proteins, termed defective ribosomal products. Most of the data supporting this concept come from studies in which inhibitors of protein synthesis were found to rapidly block Ag presentation even when cells contained a pool of mature proteins. However, these data only indirectly address the origin of presented peptides, and in most studies, the contribution of mature functional proteins to the class I peptide pool has not been directly quantified. In this report, we address the efficiency and contribution of mature proteins using a tetracycline-inducible system to express Ags that are conditionally stabilized upon ligand binding. This system circumvents the use of general inhibitors of protein synthesis to control Ag expression. Moreover, by controlling Ag stabilization, we could investigate whether the degradation of mature Ags contributed to Ag presentation at early and/or late time points. We show that mature proteins are the major contributor of peptides presented on class I for two distinct antigenic constructs. Furthermore, our data show that the protein synthesis inhibitors used previously to test the contribution of defective proteins actually block Ag presentation in ways that are independent from blocking Ag synthesis. These data suggest that for the constructs we have analyzed, mature functional proteins, rather than defective ribosomal products, are the predominant source of MHC class I-presented peptides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.