ArticleZeitschrift fur Parasitenkunde (Berlin, Germany)1985
Isolation of specific antigens from Angiostrongylus cantonensis. 1. Preparative flatbed isoelectric focusing.
Article in Zeitschrift fur Parasitenkunde (Berlin, Germany), 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Identification of stage-specific proteins of Angiostrongylus vasorum (Baillet, 1866) Kamensky.Parasitology research · 2008Article
- Angiostrongylus cantonensis: immunoblot analysis of the antigens recognized by rats.Parasitology research · 1987Article
- Isolation of specific antigens from Angiostrongylus cantonensis. 2. By affinity chromatography.Zeitschrift fur Parasitenkunde (Berlin, Germany) · 1986Article
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2 authors.
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Abstract
Electrophoresis on SDS gel and analytical isoelectric focusing showed that a crude extract of Angiostrongylus cantonensis consisted of at least 40 protein components with molecular weights ranging from 13 000-70 000 and isoelectric points of pI values ranging from 3.7-10.0. Crossed-immunoelectrophoresis with a hyperimmune antiserum to A. cantonensis showed at least 40 different antigenic components in the crude worm extract which were cross-reactive with those of Ascaris suum, Metastrongylus apri and Toxocara canis. Using preparative isoelectric focusing, the somatic worm preparation was divided into 13 equal fractions, of which 3, 4 and 5, with pI values of 3.7, 4.0 and 4.45 respectively, were later shown by immunoelectrophoretic techniques and enzyme-linked immunosorbent assay to contain antigens specific to A. cantonensis.
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