Evidence map›Paper›PMID 24174129›Full record

ArticleStem cell reviews and reports2014

Enhanced medial collateral ligament healing using mesenchymal stem cells: dosage effects on cellular response and cytokine profile.

Erin E Saether, Connie S Chamberlain, Ellen M Leiferman, Jaclyn R Kondratko-Mittnacht, Wan Ju Li, Stacey L Brickson, Ray Vanderby

Abstract read
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In one paragraph

Article in Stem cell reviews and reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Results and insights from a phase I clinical trial of Lomecel-B for Alzheimer's disease.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2023
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  14. Tendon and Ligament Healing and Current Approaches to Tendon and Ligament Regeneration.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Erin E SaetherDepartment of Orthopedics and Rehabilitation, University of Wisconsin, 1111 Highland Ave., 5th Floor WIMR, Madison, WI, 53705, USA.
Connie S Chamberlain
Ellen M Leiferman
Jaclyn R Kondratko-Mittnacht
Wan Ju Li
Stacey L Brickson
Ray Vanderby

Funding

Modulation of the Immune System to Improve Ligament/Ligament Graft HealingR01AR059916 · NIAMS · UNIVERSITY OF WISCONSIN-MADISON · PI MURPHY, WILLIAM L., VANDERBY, RAY · 2010 to 2014
$1.4M
NIAMS NIH HHS AR059916NIAMS NIH HHS R01 AR059916
6 · The paper itself

Abstract

Mesenchymal stem cells (MSCs) have potential therapeutic applications for musculoskeletal injuries due to their ability to differentiate into several tissue cell types and modulate immune and inflammatory responses. These immune-modulatory properties were examined in vivo during early stage rat medial collateral ligament healing. Two different cell doses (low dose 1 × 10(6) or high dose 4 × 10(6) MSCs) were administered at the time of injury and compared with normal ligament healing at days 5 and 14 post-injury. At both times, the high dose MSC group demonstrated a significant decrease in M2 macrophages compared to controls. At day 14, fewer M1 macrophages were detected in the low dose group compared to the high dose group. These results, along with significant changes in procollagen I, proliferating cells, and endothelialization suggest that MSCs can alter the cellular response during healing in a dose-dependent manner. The higher dose ligaments also had increased expression of several pro-inflammatory cytokines at day 5 (IL-1β, IFNγ, IL-2) and increased expression of IL-12 at day 14. Mechanical testing at day 14 revealed increased failure strength and stiffness in low dose ligaments compared to controls. Based on these improved mechanical properties, MSCs enhanced functional healing when applied at a lower dose. Different doses of MSCs uniquely affected the cellular response and cytokine expression in healing ligaments. Interestingly, the lower dose of cells proved to be most effective in improving functional properties.

Indexed as

Mesenchymal Stem Cell TransplantationWound HealingAnimalsCells, CulturedCytokinesMaleMedial Collateral Ligament, KneeMesenchymal Stem CellsRatsRats, WistarCytokines

Identifiers

PMID24174129
PMCPMC3946728

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.