ArticleJournal of the American Society for Mass Spectrometry2014
Intact microRNA analysis using high resolution mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- MicroRNA Significance in Cancer: An Updated Review on Diagnostic, Prognostic, and Therapeutic Perspectives.EJIFCC · 2024Review
- Analysis of RNA Sequences and Modifications Using NASE.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Two-step formulation of magnetic nanoprobes for microRNA capture.RSC advances · 2022Article
- A novel method to purify small RNAs from human tissues for methylation analysis by LC-MS/MS.Frontiers in molecular biosciences · 2022Article
- A call for the standardised reporting of factors affecting the exogenous loading of extracellular vesicles with therapeutic cargos.Advanced drug delivery reviews · 2021Review
- Utilization of chromogenic enzyme substrates for signal amplification in multiplexed detection of biomolecules using surface mass spectrometry.Sensors and actuators. B, Chemical · 2021Article
- Unraveling the RNA modification code with mass spectrometry.Molecular omics · 2020Article
- A rapid and reliable CE-LIF method for the quantitative analysis of miRNA-497 in plasma and organs and its application to a pharmacokinetic and biodistribution study.RSC advances · 2020Article
- A computational platform for high-throughput analysis of RNA sequences and modifications by mass spectrometry.Nature communications · 2020Article
- MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing.Frontiers in microbiology · 2020Review
- Quantification of MicroRNAs by Coupling Cyclic Enzymatic Amplification with Microfluidic Voltage-Assisted Liquid Desorption Electrospray Ionization Mass Spectrometry.Analytical chemistry · 2018Article
- Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry.Journal of visualized experiments : JoVE · 2016Article
- Novel glycylated sugar alcohols protect ESC-specific microRNAs from degradation in iPS cells.Nucleic acids research · 2016Article
- Mass spectrometry of modified RNAs: recent developments.The Analyst · 2016Review
- Novel RNA modifications in the nervous system: form and function.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Review
- Analytical tools and current challenges in the modern era of neuroepigenomics.Nature neuroscience · 2014Review
- Present and Future Applications of High Resolution Mass Spectrometry in the Clinic.Discoveries (Craiova, Romania) · 2014Article
- The identification and characterization of non-coding and coding RNAs and their modified nucleosides by mass spectrometry.RNA biology · 2014Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
MicroRNAs (miRNAs) are small single-stranded non-coding RNAs that post-transcriptionally regulate gene expression, and play key roles in the regulation of a variety of cellular processes and in disease. New tools to analyze miRNAs will add understanding of the physiological origins and biological functions of this class of molecules. In this study, we investigate the utility of high resolution mass spectrometry for the analysis of miRNAs through proof-of-concept experiments. We demonstrate the ability of mass spectrometry to resolve and separate miRNAs and corresponding 3' variants in mixtures. The mass accuracy of the monoisotopic deprotonated peaks from various miRNAs is in the low ppm range. We compare fragmentation of miRNA by collision-induced dissociation (CID) and by higher-energy collisional dissociation (HCD) which yields similar sequence coverage from both methods but additional fragmentation by HCD versus CID. We measure the linear dynamic range, limit of detection, and limit of quantitation of miRNA loaded onto a C18 column. Lastly, we explore the use of data-dependent acquisition of MS/MS spectra of miRNA during online LC-MS and demonstrate that multiple charge states can be fragmented, yielding nearly full sequence coverage of miRNA on a chromatographic time scale. We conclude that high resolution mass spectrometry allows the separation and measurement of miRNAs in mixtures and a standard LC-MS setup can be adapted for online analysis of these molecules.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.