ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1995
Identification of cytoplasms using the polymerase chain reaction to aid in the extraction of maintainer lines from open-pollinated populations of onion.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
11 citing papers in PubMed.
- Two-Step Identification of N-, S-, R- and T-Cytoplasm Types in Onion Breeding Lines Using High-Resolution Melting (HRM)-Based Markers.International journal of molecular sciences · 2023Article
- Construction of a high-resolution genetic map and identification of single nucleotide polymorphism markers relevant to flower stalk height in onion.Frontiers in plant science · 2023Article
- Molecular marker-based characterization of cytoplasm and restorer of male sterility (Ms) locus in commercially grown onions in India.Molecular biology reports · 2022Article
- Identification of a gene responsible for cytoplasmic male-sterility in onions (Allium cepa L.) using comparative analysis of mitochondrial genome sequences of two recently diverged cytoplasms.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- High-resolution tyramide-FISH mapping of markers tightly linked to the male-fertility restoration (Ms) locus of onion.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Identification of candidate genes associated with fertility restoration of cytoplasmic male-sterility in onion (Allium cepa L.) using a combination of bulked segregant analysis and RNA-seq.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Garlic (Allium sativum L.) fertility: transcriptome and proteome analyses provide insight into flower and pollen development.Frontiers in plant science · 2015Article
- Identification of highly variable chloroplast sequences and development of cpDNA-based molecular markers that distinguish four cytoplasm types in radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Identification of a novel chimeric gene, orf725, and its use in development of a molecular marker for distinguishing among three cytoplasm types in onion (Allium cepa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Identification of sterility-inducing cytoplasms in rye using the plasmotype-genotype interaction test and newly developed SCAR markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Biodiversity assessment based on cpDNA and crossability analysis in selected species of Allium subgenus Rhizirideum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
S-cytoplasm is the most common source of cytoplasmic-genic male sterility (CMS) used to produce hybrid-onion seed. Identification of the cytoplasm of a single plant takes from 4 to 8 years and is complicated by the segregation of a nuclear gene that restores fertility. Although CMS in onion may be due to an incompatibility between the mitochondrial and nuclear genomes, Southern analyses of DNA from individual plants from crosses of S- and N-cytoplasmic plants supported maternal inheritance of the chloroplast and mitochondrial DNA and, therefore, polymorphisms in the chloroplast DNA may be used to classify cytoplasms. Amplification by the polymerase chain reaction of a fragment that carries an autapomorphic 100-bp insertion in the chloroplast DNA of N-cytoplasm offers a significantly quicker and cheaper alternative to crossing or Southern analysis. Molecular characterization of N- and S-cytoplasms and frequencies of the nuclear non-restoring allele allow onion breeders to determine the proportion of plants in open-pollinated populations that maintain CMS and can significantly reduce the investment required to identify individual maintainer plants.
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Registered trials
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