ArticleMolecular biology of the cell2014
Substrate stiffness regulates solubility of cellular vimentin.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 84 citations in OpenAlex.
- Fibrillar adhesion dynamics govern the timescales of nuclear mechano-response via the vimentin cytoskeleton.Nature materials · 2026Article
- Continuous self-repair protects vimentin intermediate filaments from fragmentation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Vimentin undergoes liquid-liquid phase separation to form droplets which wet and stabilize actin fibers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- N-cadherin crosstalk with integrin weakens the molecular clutch in response to surface viscosity.Nature communications · 2024Article
- Mechanobiology in Metabolic Dysfunction-Associated Steatotic Liver Disease and Obesity.Current issues in molecular biology · 2024Review
- The Role of Vimentin in Human Corneal Fibroblast Spreading and Myofibroblast Transformation.Cells · 2024Article
- Theranostics application of tumor-initiating cell probe TiY in non-small cell lung cancer.Theranostics · 2023Article
- Vimentin binds to G-quadruplex repeats found at telomeres and gene promoters.Nucleic acids research · 2022Article
- Vimentin Intermediate Filaments Mediate Cell Morphology on Viscoelastic Substrates.ACS applied bio materials · 2022Article
- Cytoskeleton-mediated alterations of nuclear mechanics by extracellular mechanical signals.Biophysical journal · 2022Article
- Vimentin Inhibits Dengue Virus Type 2 Invasion of the Blood-Brain Barrier.Frontiers in cellular and infection microbiology · 2022Article
- Mechanobiology in the Comorbidities of Ehlers Danlos Syndrome.Frontiers in cell and developmental biology · 2022Review
- Mechanical programming of arterial smooth muscle cells in health and ageing.Biophysical reviews · 2021Review
- Vimentin Cytoskeleton Architecture Analysis on Polylactide and Polyhydroxyoctanoate Substrates for Cell Culturing.International journal of molecular sciences · 2021Article
- Gravity-Vector Induces Mechanical Remodeling of rMSCsFrontiers in bioengineering and biotechnology · 2021Article
- The vimentin cytoskeleton: when polymer physics meets cell biology.Physical biology · 2020Article
- Keratocyte mechanobiology.Experimental eye research · 2020Review
- Mechanical and Non-Mechanical Functions of Filamentous and Non-Filamentous Vimentin.BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Review
- Unidirectional Regulation of Vimentin Intermediate Filaments to Caveolin-1.International journal of molecular sciences · 2020Article
- Evaluation of active Rac1 levels in cancer cells: A case of misleading conclusions from immunofluorescence analysis.The Journal of biological chemistry · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The intermediate filament protein vimentin is involved in the regulation of cell behavior, morphology, and mechanical properties. Previous studies using cells cultured on glass or plastic substrates showed that vimentin is largely insoluble. Although substrate stiffness was shown to alter many aspects of cell behavior, changes in vimentin organization were not reported. Our results show for the first time that mesenchymal stem cells (hMSCs), endothelial cells, and fibroblasts cultured on different-stiffness substrates exhibit biphasic changes in vimentin detergent solubility, which increases from nearly 0 to 67% in hMSCs coincident with increases in cell spreading and membrane ruffling. When imaged, the detergent-soluble vimentin appears to consist of small fragments the length of one or several unit-length filaments. Vimentin detergent solubility decreases when these cells are subjected to serum starvation, allowed to form cell-cell contacts, after microtubule disruption, or inhibition of Rac1, Rho-activated kinase, or p21-activated kinase. Inhibiting myosin or actin assembly increases vimentin solubility on rigid substrates. These data suggest that in the mechanical environment in vivo, vimentin is more dynamic than previously reported and its assembly state is sensitive to stimuli that alter cellular tension and morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.