ArticleMolecular biology of the cell2014
Direct observation of microtubule pushing by cortical dynein in living cells.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Multiscale temporal tuning of force generation complex machinery governs cortical microtubule interactions during the first mitotic division inbioRxiv : the preprint server for biology · 2026Article
- Cell adhesion and spreading on fluid membranes through microtubules-dependent mechanotransduction.Nature communications · 2025Article
- Differential regulation of MAP2 by phosphorylation events in proline-rich versus C-terminal domains.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Establishing neuronal polarity: microtubule regulation during neurite initiation.Oxford open neuroscience · 2022Review
- Centering and Shifting of Centrosomes in Cells.Cells · 2020Review
- Unconventional Roles of Cytoskeletal Mitotic Machinery in Neurodevelopment.Trends in cell biology · 2019Review
- Polarity Sorting of Microtubules in the Axon.Trends in neurosciences · 2018Review
- Polarity sorting of axonal microtubules: a computational study.Molecular biology of the cell · 2017Article
- Moonlighting Motors: Kinesin, Dynein, and Cell Polarity.Trends in cell biology · 2017Review
- Crosstalk of cell polarity signaling pathways.Protoplasma · 2017Review
- Article
- Ciliobrevins as tools for studying dynein motor function.Frontiers in cellular neuroscience · 2015Review
- Cytoskeletal self-organization in neuromorphogenesis.BioarchitectureArticle
Corrections and comments
- Commented on by
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules are under the influence of forces mediated by cytoplasmic dynein motors associated with the cell cortex. If such microtubules are free to move, they are rapidly transported inside cells. Here we directly observe fluorescent protein-labeled cortical dynein speckles and motile microtubules. We find that several dynein complex subunits, including the heavy chain, the intermediate chain, and the associated dynactin subunit Dctn1 (also known as p150glued) form spatially resolved, dynamic speckles at the cell cortex, which are preferentially associated with microtubules. Measurements of bleaching and dissociation kinetics at the cell cortex reveal that these speckles often contain multiple labeled dynein heavy-chain molecules and turn over rapidly within seconds. The dynamic behavior of microtubules, such as directional movement, bending, or rotation, is influenced by association with dynein speckles, suggesting a direct physical and functional interaction. Our results support a model in which rapid turnover of cell cortex-associated dynein complexes facilitates their search to efficiently capture and push microtubules directionally with leading plus ends.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.