ArticleProceedings of the National Academy of Sciences of the United States of America2013
Structures of intermediate transport states of ZneA, a Zn(II)/proton antiporter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Structural insights into a substrate translocation pathway revealed by the RND efflux pump complex MexJK fromScience advances · 2026Article
- The Resistance-Nodulation-Division efflux pump EefABC is highly conserved within lineages ofMicrobial genomics · 2026Article
- Manganese Efflux Achieved by MetA and MetB Affects Oxidative Stress Resistance and Iron Homeostasis in Riemerella anatipestifer.Applied and environmental microbiology · 2023Article
- Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes.Analytical chemistry · 2022Article
- The TbD1 Locus Mediates a Hypoxia-Induced Copper Response inFrontiers in microbiology · 2022Article
- Structural and Functional Diversity of Resistance-Nodulation-Cell Division Transporters.Chemical reviews · 2021Review
- Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.Chemical reviews · 2021Review
- Article
- A "Drug Sweeping" State of the TriABC Triclosan Efflux Pump from Pseudomonas aeruginosa.Structure (London, England : 1993) · 2021Article
- Adaptation ofMicroorganisms · 2021Article
- Cryo-EM as a tool to study bacterial efflux systems and the membrane proteome.Faculty reviews · 2021Review
- Structural basis of ion transport and inhibition in ferroportin.Nature communications · 2020Article
- Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex.Nature communications · 2020Article
- Article
- Cobalt Resistance via Detoxification and Mineralization in the Iron-Reducing BacteriumFrontiers in microbiology · 2020Article
- A convenient protein library for spectroscopic calibrations.Computational and structural biotechnology journal · 2020Article
- MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- MmpL Proteins in Physiology and Pathogenesis ofMicroorganisms · 2019Review
- Unintentional Genomic Changes EndowGenes · 2018Article
- BpeB, a major resistance-nodulation-cell division transporter from Burkholderia cenocepacia: construct design, crystallization and preliminary structural analysis.Acta crystallographica. Section F, Structural biology communications · 2018Article
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Authors and funding
12 authors.
Funding
Abstract
Efflux pumps belonging to the ubiquitous resistance-nodulation-cell division (RND) superfamily transport substrates out of cells by coupling proton conduction across the membrane to a conformationally driven pumping cycle. The heavy metal-resistant bacteria Cupriavidus metallidurans CH34 relies notably on as many as 12 heavy metal efflux pumps of the RND superfamily. Here we show that C. metallidurans CH34 ZneA is a proton driven efflux pump specific for Zn(II), and that transport of substrates through the transmembrane domain may be electrogenic. We report two X-ray crystal structures of ZneA in intermediate transport conformations, at 3.0 and 3.7 Å resolution. The trimeric ZneA structures capture protomer conformations that differ in the spatial arrangement and Zn(II) occupancies at a proximal and a distal substrate binding site. Structural comparison shows that transport of substrates through a tunnel that links the two binding sites, toward an exit portal, is mediated by the conformation of a short 14-aa loop. Taken together, the ZneA structures presented here provide mechanistic insights into the conformational changes required for substrate efflux by RND superfamily transporters.
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