ArticleClinical and vaccine immunology : CVI2014
Blood collection tubes influence serum ficolin-1 and ficolin-2 levels.
Article in Clinical and vaccine immunology : CVI, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- Pitfalls in complement analysis: A systematic literature review of assessing complement activation.Frontiers in immunology · 2022Pooled it
- Low invasiveness of pneumococcal serotype 11A is linked to ficolin-2 recognition of O-acetylated capsule epitopes and lectin complement pathway activation.The Journal of infectious diseases · 2014Pooled it
- Trial
- Heparin-coated cardiopulmonary bypass circuits selectively deplete the pattern recognition molecule ficolin-2 of the lectin complement pathway in vivo.Clinical and experimental immunology · 2015Trial
- Integrated and high-throughput method to collect, store, recover, and manage microbial isolates in mini-arrays.Microbiology spectrum · 2025Article
- Divergent host humoral innate immune response to the smooth-to-rough adaptation ofFrontiers in cellular and infection microbiology · 2025Article
- Complement proteins in axial spondyloarthritis: associations with disease activity and TNFi treatment response in a multicentre RCT.Rheumatology advances in practice · 2025Article
- Article
- Association of low ficolin-2 concentration in cord serum with respiratory distress syndrome in preterm newborns.Frontiers in immunology · 2023Article
- Ficolin-2 Lectin Complement Pathway Mediates Capsule-Specific Innate Immunity Against Invasive Pneumococcal Disease.Frontiers in immunology · 2022Article
- Associations of ficolins and mannose-binding lectin with acute myeloid leukaemia in adults.Scientific reports · 2020Article
- Classical and lectin complement pathways and markers of inflammation for investigation of susceptibility to infections among healthy older adults.Immunity & ageing : I & A · 2020Article
- Associations of Ficolins With Hematological Malignancies in Patients Receiving High-Dose Chemotherapy and Autologous Hematopoietic Stem Cell Transplantations.Frontiers in immunology · 2019Article
- Lectin pathway factors in patients suffering from juvenile idiopathic arthritis.Immunology and cell biology · 2017Article
- Article
- Rapid and efficient purification of ficolin-2 using a disposable CELLine bioreactor.Journal of immunological methods · 2015Article
- Potential role of the lectin pathway of complement in the pathogenesis and disease manifestations of systemic sclerosis: a case-control and cohort study.Arthritis research & therapy · 2014Article
- Commercially available complement component-depleted sera are unexpectedly codepleted of ficolin-2.Clinical and vaccine immunology : CVI · 2014Article
- Evaluation of anticoagulants for serologic assays of cholera vaccination.Clinical and vaccine immunology : CVI · 2014Article
- Interferences from blood collection tube components on clinical chemistry assays.Biochemia medica · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The ficolins are members of a recently discovered family of host innate opsonins that can activate the lectin pathway of complement. The ficolins bind many ligands, although they are typically described as binding acetylated sugars. Ficolin-1 (M-ficolin) and ficolin-2 (L-ficolin) are known to bind Streptococcus pneumoniae serotypes 19C and 11A, respectively. While studying the binding of ficolins to pneumococci, we found variations in ficolin-2 binding among serum samples collected in different types of blood collection tubes. Plastic tubes, which contain a silica clot activator, yielded sera with reduced ficolin-2 binding and apparent ficolin-2 levels. We found that the silica clot activator eluted from plastic red-top tubes inhibited ficolin-2 ligand binding, while other related proteins, like mannose-binding lectin (MBL) and ficolin-1, were not affected. These tube types did not affect the concentrations of other related opsonins (C1q, MBL, or ficolin-3 [H-ficolin]). Interestingly, we also found that ficolin-1 levels were increased 2- to 3-fold in plastic serum separator tubes compared to the increases in other tube types. These findings have implications for future ficolin-1 and ficolin-2 studies, as proper sample collection and handling are essential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.