ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1995
High-resolution flow karyotyping and chromosome sorting in Vicia faba lines with standard and reconstructed karyotypes.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- Genomic regions associated with chocolate spot (Molecular breeding : new strategies in plant improvement · 2022Article
- Flow karyotyping of wheat-Frontiers in plant science · 2022Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Development of new genetic resources for faba bean (Vicia faba L.) breeding through the discovery of gene-based SNP markers and the construction of a high-density consensus map.Scientific reports · 2020Article
- Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting.The plant genome · 2019Article
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Unlocking the barley genome by chromosomal and comparative genomics.The Plant cell · 2011Article
- Chromosomal DNA content of sweet pepper determined by association of cytogenetic and cytometric tools.Plant cell reports · 2008Article
- Chromosome-based genomics in the cereals.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Review
- Flow cytogenetics and plant genome mapping.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Review
- Flow karyotypes and chromosomal DNA contents of genus Triticum species and rye (Secale cereale).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Article
- Chromosome sorting and PCR-based physical mapping in pea (Pisum sativum L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2002Article
- Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1999Article
- Construction of chromosome-specific DNA libraries covering the whole genome of field bean (Vicia faba L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1996Article
- Preparation of pea (Pisum sativum L.) chromosome and nucleus suspensions from single root tips.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flow cytometric analysis has been performed on chromosomes isolated from formaldehyde-fixed root tips in a Vicia faba (2n = 12) line with a standard (wild-type) karyotype and in six V. faba translocation lines with reconstructed karyotypes. The resolution of individual chromosome types on histograms of chromosome fluorescence intensity (flow karyotypes) depended on the type of fluorochrome used for chromosome staining. The highest degree of resolution was achieved with 4',6-diamidino-2-phenylindole (DAPI). The lower resolution obtained after staining with mithramycin A (MIT) and propidium iodide (PI) was probably due to the sensitivity of these stains to changes in chromatin structure induced by formaldehyde fixation. After the staining with DAPI, only 1 chromosome type could be discriminated in the line with a standard karyotype. In the translocation lines, the number of chromosome types resolved on flow karyotypes ranged from 2 in the G and the ACB lines to all (6) chromosome types in the EFK and EF lines. Refined flow karyotyping permitted the sorting of a total of 15 different chromosome types from five of the translocation lines. It is expected that flow sorting of chromosomes from reconstructed karyotypes will become a powerful tool in the study of nuclear genome organisation in V. faba.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.