ArticleEpigenetics2014
A unique epigenetic signature is associated with active DNA replication loci in human embryonic stem cells.
Article in Epigenetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Genome-wide identification of replication fork stalling/pausing sites and the interplay between RNA Pol II transcription and DNA replication progression.Genome biology · 2024Article
- TFIIIC as a Potential Epigenetic Modulator of Histone Acetylation in Human Stem Cells.International journal of molecular sciences · 2023Article
- Genomic methods for measuring DNA replication dynamics.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Review
- Protein Subdomain Enrichment ofFrontiers in cardiovascular medicine · 2020Article
- Targeting RNA helicase DDX3 in stem cell maintenance and teratoma formation.Genes & cancer · 2019Article
- KAT3B-p300 and H3AcK18/H3AcK14 levels are prognostic markers for kidney ccRCC tumor aggressiveness and target of KAT inhibitor CPTH2.Clinical epigenetics · 2018Article
- Dose-response analysis of epigenetic, metabolic, and apical endpoints after short-term exposure to experimental hepatotoxicants.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2017Article
- Exploitation of EP300 and CREBBP Lysine Acetyltransferases by Cancer.Cold Spring Harbor perspectives in medicine · 2017Review
- Deterministic transfection drives efficient nonviral reprogramming and uncovers reprogramming barriers.Nanomedicine : nanotechnology, biology, and medicine · 2016Article
- The Commercial Antibodies Widely Used to Measure H3 K56 Acetylation Are Non-Specific in Human and Drosophila Cells.PloS one · 2016Article
- Distinct epigenetic features of differentiation-regulated replication origins.Epigenetics & chromatin · 2016Article
- The chromatin environment shapes DNA replication origin organization and defines origin classes.Genome research · 2015Article
- Positive feedback regulation of type I IFN production by the IFN-inducible DNA sensor cGAS.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Positive feedback regulation of type I interferon by the interferon-stimulated gene STING.EMBO reports · 2015Article
- Cell cycle-dependent changes in H3K56ac in human cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Histone-modifying enzymes, histone modifications and histone chaperones in nucleosome assembly: Lessons learned from Rtt109 histone acetyltransferases.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
The cellular epigenetic landscape changes as pluripotent stem cells differentiate to somatic cells or when differentiated cells transform to a cancerous state. These epigenetic changes are commonly correlated with differences in gene expression. Whether active DNA replication is also associated with distinct chromatin environments in these developmentally and phenotypically diverse cell types has not been known. Here, we used BrdU-seq to map active DNA replication loci in human embryonic stem cells (hESCs), normal primary fibroblasts and a cancer cell line, and correlated these maps to the epigenome. In all cell lines, the majority of BrdU peaks were enriched in euchromatin and at DNA repetitive elements, especially at microsatellite repeats, and coincided with previously determined replication origins. The most prominent BrdU peaks were shared between all cells but a sizable fraction of the peaks were specific to each cell type and associated with cell type-specific genes. Surprisingly, the BrdU peaks that were common to all cell lines were associated with H3K18ac, H3K56ac, and H4K20me1 histone marks only in hESCs but not in normal fibroblasts or cancer cells. Depletion of the histone acetyltransferases for H3K18 and H3K56 dramatically decreased the number and intensity of BrdU peaks in hESCs. Our data reveal a unique epigenetic signature that distinguishes active replication loci in hESCs from normal somatic or malignant cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.