Evidence map›Paper›PMID 24171554›Full record

ArticleNano letters2013

Nanopore-based identification of individual nucleotides for direct RNA sequencing.

Mariam Ayub, Steven W Hardwick, Ben F Luisi, Hagan Bayley

Abstract read
In one paragraph

Article in Nano letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
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  8. Article
  9. Nanopore sensing: A physical-chemical approach.Biochimica et biophysica acta. Biomembranes · 2021
    Review
  10. Review
  11. Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
  18. High-throughput determination of RNA structures.Nature reviews. Genetics · 2018
    Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mariam AyubDepartment of Chemistry, University of Oxford , Oxford OX1 3TA, United Kingdom.
Steven W Hardwick
Ben F Luisi
Hagan Bayley

Funding

Single-Molecule DNA Sequencing with Engineered NanoporesR01HG003709 · NHGRI · SCRIPPS RESEARCH INSTITUTE, THE · PI GHADIRI, M. REZA · 2005 to 2017
$13.2M
NHGRI NIH HHS R01 HG003709Wellcome Trust
6 · The paper itself

Abstract

We describe a label-free ribobase identification method, which uses ionic current measurement to resolve ribonucleoside monophosphates or diphosphates in α-hemolysin protein nanopores containing amino-cyclodextrin adapters. The accuracy of base identification is further investigated through the use of a guanidino-modified adapter. On the basis of these findings, an exosequencing approach is envisioned in which a processive exoribonuclease (polynucleotide phosphorylase) presents sequentially cleaved ribonucleoside diphosphates to a nanopore.

Indexed as

NanoporesSequence Analysis, RNABase SequenceHemolysin ProteinsNanostructuresNucleotidesHemolysin ProteinsNucleotides

Identifiers

PMID24171554
PMCPMC3899427

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.