ArticleJournal of the American Chemical Society2013
Discovery and synthetic refactoring of tryptophan dimer gene clusters from the environment.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Benchmarking methods for measuring biosynthetic gene cluster similarity and determination of gene cluster families.Bioinformatics (Oxford, England) · 2025Article
- Advances, opportunities, and challenges in methods for interrogating the structure activity relationships of natural products.Natural product reports · 2024Review
- Structure of methyltransferase RedM that forms the dimethylpyrrolinium of the bisindole reductasporine.The Journal of biological chemistry · 2024Article
- Hydroxytryptophan biosynthesis by a family of heme-dependent enzymes in bacteria.Nature chemical biology · 2023Article
- Draft genome sequence of potato crop bacterial isolates and nanoparticles-intervention for the induction of secondary metabolites biosynthesis.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2023Article
- Cadasides, Calcium-Dependent Acidic Lipopeptides from the Soil Metagenome That Are Active against Multidrug-Resistant Bacteria.Journal of the American Chemical Society · 2019Article
- Concepts and Methods to Access Novel Antibiotics from Actinomycetes.Antibiotics (Basel, Switzerland) · 2018Review
- Phylogeny-guided (meta)genome mining approach for the targeted discovery of new microbial natural products.Journal of industrial microbiology & biotechnology · 2017Review
- Biosynthetic Functional Gene Analysis of Bis-Indole Metabolites from 25D7, a Clone Derived from a Deep-Sea Sediment Metagenomic Library.Marine drugs · 2016Article
- Culture-independent discovery of natural products from soil metagenomes.Journal of industrial microbiology & biotechnology · 2016Review
- A Rebeccamycin Analog Provides Plasmid-Encoded Niche Defense.Journal of the American Chemical Society · 2015Article
- Discovery of microbial natural products by activation of silent biosynthetic gene clusters.Nature reviews. Microbiology · 2015Review
- Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Expansion of bisindole biosynthetic pathways by combinatorial construction.ACS synthetic biology · 2015Article
- Targeted metagenomics: finding rare tryptophan dimer natural products in the environment.Journal of the American Chemical Society · 2015Article
- Mining the metabiome: identifying novel natural products from microbial communities.Chemistry & biology · 2014Review
- eSNaPD: a versatile, web-based bioinformatics platform for surveying and mining natural product biosynthetic diversity from metagenomes.Chemistry & biology · 2014Article
- Metagenomic small molecule discovery methods.Current opinion in microbiology · 2014Article
- Characterization of an environmental DNA-derived gene cluster that encodes the bisindolylmaleimide methylarcyriarubin.Chembiochem : a European journal of chemical biology · 2014Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
Here we investigate bacterial tryptophan dimer (TD) biosynthesis by probing environmental DNA (eDNA) libraries for chromopyrrolic acid (CPA) synthase genes. Functional and bioinformatics analyses of TD clusters indicate that CPA synthase gene sequences diverge in concert with the functional output of their respective clusters, making this gene a powerful tool for guiding the discovery of novel TDs from the environment. Twelve unprecedented TD biosynthetic gene clusters that can be arranged into five groups (A-E) based on their ability to generate distinct TD core substructures were recovered from eDNA libraries. Four of these groups contain clusters from both cultured and culture independent studies, while the remaining group consists entirely of eDNA-derived clusters. The complete synthetic refactoring of a representative gene cluster from the latter eDNA specific group led to the characterization of the erdasporines, cytotoxins with a novel carboxy-indolocarbazole TD substructure. Analysis of CPA synthase genes in crude eDNA suggests the presence of additional TD gene clusters in soil environments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.