ArticlePlant cell reports2014
Virus-induced gene silencing for comparative functional studies in Gladiolus hybridus.
Article in Plant cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- A VIGS-based leaf disc method reveals ethylene's crucial role in hydrangea leaf spot resistance.Plant cell reports · 2025Article
- Agrobacterium-mediated transformation for virus-induced gene silencing (VIGS) in Castilleja tenuiflora: Cte-chlH and Cte-PDS.Plant cell reports · 2025Article
- Efficient Virus-Induced Gene Silencing (VIGS) Method for Discovery of Resistance Genes in Soybean.Plants (Basel, Switzerland) · 2025Article
- In Vitro Culture Technology and Advanced Biotechnology Tools Toward Improvement in Gladiolus (Gladiolus species): Present Scenario and Future Prospects.Molecular biotechnology · 2024Review
- Tobacco rattle virus-based virus-induced gene silencing (VIGS) as an aid for functional genomics in Saffron (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Establishment of a Homologous Silencing System with Intact-Plant Infiltration and Minimized Operation for Studying Gene Function in Herbaceous Peonies.International journal of molecular sciences · 2024Article
- Inoculation of Maize with Sugarcane Mosaic Virus Constructs and Application for RNA Interference in Fall Armyworms.Bio-protocol · 2023Article
- Priming defense by transiently suppressing plant susceptibility genes in ornamental crops: A novel strategy for post-harvest diseases management.Frontiers in plant science · 2022Article
- Antagonism between abscisic acid and gibberellin regulates starch synthesis and corm development in Gladiolus hybridus.Horticulture research · 2021Article
- GhNAC83 inhibits corm dormancy release by regulating ABA signaling and cytokinin biosynthesis in Gladiolus hybridus.Journal of experimental botany · 2019Article
- Tobacco rattle virus-inducedPeerJ · 2018Article
- Sugarcane Mosaic Virus-Based Gene Silencing inIranian journal of biotechnology · 2017Article
- Virus-induced gene silencing (VIGS) inPlant biotechnology (Tokyo, Japan) · 2016Article
- The NPR1 homolog GhNPR1 plays an important role in the defense response of Gladiolus hybridus.Plant cell reports · 2015Article
- Gladiolus hybridus ABSCISIC ACID INSENSITIVE 5 (GhABI5) is an important transcription factor in ABA signaling that can enhance Gladiolus corm dormancy and Arabidopsis seed dormancy.Frontiers in plant science · 2015Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional analysis of genes in gladiolus has previously been impractical due to the lack of an efficient stable genetic transformation method. However, virus-induced gene silencing (VIGS) is effective in some plants which are difficult to transform through other methods. Although the Tobacco rattle virus (TRV)-based VIGS system has been developed and used for verifying gene functions in diverse plants, an appropriate TRV-VIGS approach for gladiolus has not been established yet. In this report we describe the first use of the TRV-VIGS system for gene silencing in gladiolus. Vacuum infiltration of cormels and young plants with the GhPDS-VIGS vector effectively down-regulated the PHYTOENE DESATURASE ortholog GhPDS gene and also resulted in various degrees of photobleaching in Gladiolus hybridus. The reduction in GhPDS expression was tested after TRV-based vector infection using real-time RT-PCR. In addition, the progress of TRV infection was detected by fluorescence visualization using a pTRV2: CP-GFP vector. In conclusion, the TRV-mediated VIGS described here will be an effective gene function analysis mechanism in gladiolus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.