ArticleJournal of tissue engineering and regenerative medicine2016
Dynamic three-dimensional micropatterned cell co-cultures within photocurable and chemically degradable hydrogels.
Article in Journal of tissue engineering and regenerative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Engineering Functional Cardiac Tissues for Regenerative Medicine Applications.Current cardiology reports · 2019Pooled it
- Engineering autoimmune disease models using organoids: Harnessing microenvironmental engineering for precision medicine and immunological recapitulation.Bioengineering & translational medicine · 2026Review
- From flow to form: structuring and patterning hydrogels using microfluidic approaches.Lab on a chip · 2026Review
- Addressing neuroinflammation in human induced pluripotent stem cell-derived central nervous system neurospheroids.iScience · 2025Article
- Advances in Conductive Biomaterials for Cardiac Tissue Engineering: Design, Fabrication, and Functional Integration.Polymers · 2025Review
- An automated high-content screening and assay platform for the analysis of spheroids at subcellular resolution.PloS one · 2024Article
- Keeping It Organized: Multicompartment Constructs to Mimic Tissue Heterogeneity.Advanced healthcare materials · 2023Review
- Comparative Study of Two Common In Vitro Models for the Pancreatic Islet with MIN6.Tissue engineering and regenerative medicine · 2023Article
- Cell3: a new vision for study of the endomembrane system in mammalian cells.Bioscience reports · 2021Review
- Core-shell patterning of synthetic hydrogelsChemical science · 2018Article
- Multiorgan Microphysiological Systems for Drug Development: Strategies, Advances, and Challenges.Advanced healthcare materials · 2018Review
- A Tissue Engineered Model of Aging: Interdependence and Cooperative Effects in Failing Tissues.Scientific reports · 2017Article
- Cell-friendly inverse opal-like hydrogels for a spatially separated co-culture system.Macromolecular rapid communications · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In this paper we report on the development of dynamically controlled three-dimensional (3D) micropatterned cellular co-cultures within photocurable and chemically degradable hydrogels. Specifically, we generated dynamic co-cultures of micropatterned murine embryonic stem (mES) cells with human hepatocellular carcinoma (HepG2) cells within 3D hydrogels. HepG2 cells were used due to their ability to direct the differentiation of mES cells through secreted paracrine factors. To generate dynamic co-cultures, mES cells were first encapsulated within micropatterned photocurable poly(ethylene glycol) (PEG) hydrogels. These micropatterned cell-laden PEG hydrogels were subsequently surrounded by calcium alginate (Ca-Alg) hydrogels containing HepG2 cells. After 4 days, the co-culture step was halted by exposing the system to sodium citrate solution, which removed the alginate gels and the encapsulated HepG2 cells. The encapsulated mES cells were then maintained in the resulting cultures for 16 days and cardiac differentiation was analysed. We observed that the mES cells that were exposed to HepG2 cells in the co-cultures generated cells with higher expression of cardiac genes and proteins, as well as increased spontaneous beating. Due to its ability to control the 3D microenvironment of cells in a spatially and temporally regulated manner, the method presented in this study is useful for a range of cell-culture applications related to tissue engineering and regenerative medicine. Copyright © 2013 John Wiley & Sons, Ltd.
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