ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1995
Detection of section-specific random amplified polymorphic DNA (RAPD) markers in Lilium.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- LTR-retrotransposons and inter-retrotransposon amplified polymorphism (IRAP) analysis in Lilium species.Genetica · 2015Article
- Molecular phylogeny and genetic variation in the genus Lilium native to China based on the internal transcribed spacer sequences of nuclear ribosomal DNA.Journal of plant research · 2014Article
Corrections and comments
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Authors and funding
1 author.
Funding
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Abstract
Random amplified polymorphic DNA (RAPD) markers were utilized for the identification of Lilium species and inter-specific hybrids. The optimum annealing temperature of the polymerase chain reaction (PCR) for the RAPD assay in Lilium was 54 °C, which is relatively higher than the temperature used for other genera reported by previous researchers. Among 76 primers used to amplify genomic DNA by PCR, 18 primers (24%) generated polymorphic DNA fragments in Lilium species and hybrids. Cultivars were also identified by RAPD markers. Some amplified fragments were unique to species of each section and to hybrids derived from these species; that is, they were the section-specific DNA markers. Sections, Sinomartagon, Leucolirion b, Leucolirion a and Archelirion could be identified by 6 section-specific markers amplified with five primers. Seven inter-section hybrids showed the section-specific bands of both parental sections, indicating that these markers would be useful for identifying the parental sections of inter-section hybrids.
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Registered trials
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