ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1995
Physiological aspects of genome variability in tissue culture. I. Growth phase-dependent differential DNA methylation of the carrot genome (Daucus carota L.) during primary culture.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Stress-Induced Accumulation of DcAOX1 and DcAOX2a Transcripts Coincides with Critical Time Point for Structural Biomass Prediction in Carrot Primary Cultures (Daucus carota L.).Frontiers in genetics · 2016Article
- KNOX1 is expressed and epigenetically regulated during in vitro conditions in Agave spp.BMC plant biology · 2012Article
- Epigenomic consequences of immortalized plant cell suspension culture.PLoS biology · 2008Article
- Dedifferentiation of tobacco cells is associated with ribosomal RNA gene hypomethylation, increased transcription, and chromatin alterations.Plant physiology · 2005Article
- Stress-induced cell reprogramming. A role for global genome regulation?Plant physiology · 2004Review
- Epigenetic aspects of somaclonal variation in plants.Plant molecular biology · 2000Review
- Physiological aspects of genome variability in tissue culture. II. Growth phase-dependent quantitative variability of repetitive BstNI fragments of primary cultures of Daucus carota L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1995Article
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3 authors.
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Abstract
Investigations were performed on growth phase-dependent EcoRII site-specific DNA methylation of the carrot genome during primary culture to elucidate physiological aspects of genome DNA variability in tissue culture. While DNA methylation of the root cambium and the secondary phloem and petioles of carrot leaves were strikingly different, the methylation level of the secondary phloem seemed to be independent of cultivar origin, the age of the plants and the extent of secondary root growth. As was shown earlier a change in the differentiated state of the secondary phloem by tissue culture leads to changes in genome modification. Whereas de novo methylation was observed during the first 2 weeks of growth initiation, the results presented demonstrate genome de-methylation during the transition to stationary growth indicating differential εnome methylation during different phases of culture. The presence of kinetin in the nutrient medium of the primary culture was found to be antagonistic to changes in genome modification in general. De novo methylation and subsequent de-methylation of the carrot genome are discussed as gross changes obviously essential to molecular genome differentiation during tissue culture.
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