ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1995
Abundance and characterization of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brassica napus L. (rapeseed).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Development of genome-specific SSR markers for the identification of introgressed segments of3 Biotech · 2022Article
- The first genetic map of a synthesized allohexaploid Brassica with A, B and C genomes based on simple sequence repeat markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Article
- Association of Agronomic Traits with SNP Markers in Durum Wheat (Triticum turgidum L. durum (Desf.)).PloS one · 2015Article
- A combined linkage and regional association mapping validation and fine mapping of two major pleiotropic QTLs for seed weight and silique length in rapeseed (Brassica napus L.).BMC plant biology · 2014Article
- Development of simple sequence repeat (SSR) markers of sesame (Sesamum indicum) from a genome survey.Molecules (Basel, Switzerland) · 2014Article
- Transcriptome sequencing of two parental lines of cabbage (Brassica oleracea L. var. capitata L.) and construction of an EST-based genetic map.BMC genomics · 2014Article
- Genome-wide microsatellite characterization and marker development in the sequenced Brassica crop species.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Identification of putative quantitative trait loci associated with a flavonoid related to resistance to cabbage seedpod weevil (Ceutorhynchus obstrictus) in canola derived from an intergeneric cross, Sinapis alba × Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Identification of a major QTL for silique length and seed weight in oilseed rape (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Article
- Simple sequence repeat polymorphisms (SSRPs) for evaluation of molecular diversity and germplasm classification of minor crops.Molecules (Basel, Switzerland) · 2009Review
- Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Minimising inter-laboratory variation when constructing a unified molecular database of plant varieties in an allogamous crop.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Development of a set of public SSR markers derived from genomic sequence of a rapid cycling Brassica oleracea L. genotype.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Mapping the anthocyaninless (anl) locus in rapid-cycling Brassica rapa (RBr) to linkage group R9.BMC genetics · 2007Article
- A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Plastid genome characterisation in Brassica and Brassicaceae using a new set of nine SSRs.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- The pattern of genetic diversity of Guinea-race Sorghum bicolor (L.) Moench landraces as revealed with SSR markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Conserved simple sequence repeats for the Limnanthaceae (Brassicales).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A size-fractionated library of Brassica napus L. (rapeseed), composed of 15000 clones, was screened for the presence of GA-, CA-, and GATA-simple-sequence repeats (SSRs). GA-SSRs were four- and five-fold more abundant than CA- and GATA-SSRs, respectively, and present at a frequency of approximately one SSR for every 100 kb of DNA. Following the sequencing of 124 positive clones, primer pairs were designed and evaluated for seven selected SSRs. Products were amplified in an array of individuals of B. napus, B. oleracea and B. rapa, demonstrating that the seven SSRs were conserved among species. Two SSRs were polymorphic. Among 11 accessions, the dinucleotide (GA)-repeat, B.n.9A, yielded 12 fragments, while the tetranucleotide-repeat (GATA), B.n.6A2, revealed two fragments. Automated, fluorescence-based detection of polyacrylamide gels has been employed to simultaneously increase throughput, reduce unit cost, improve analytical resolution, and expedite data acquisition of SSR analysis. Though initial financial investment and technical capabilities may prevent some from directly employing our documented approach, SSR analysis warrants further investigation as a tool in genetic studies for enhancing both the conservation and utilization of genetic resources.
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Registered trials
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