ArticleEndocrinology2013
Loss of vascular endothelial growth factor A (VEGFA) isoforms in the testes of male mice causes subfertility, reduces sperm numbers, and alters expression of genes that regulate undifferentiated spermatogonia.
Article in Endocrinology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- In vitro evaluation of cell viability and expression profile of growth factors in mouse Sertoli cells exposed to Delta-9-tetrahydrocannabinol: a mechanistic insight into the cannabinoid-induced testicular toxicity.BMC pharmacology & toxicology · 2023Article
- Impaired Testicular Function without Altering Testosterone Concentration Using an Anti-Follicular-Stimulating Hormone Receptor (Anti-FSHr) Single-Chain Variable Fragment (scFv) in Long-Tailed Macaques (Animals : an open access journal from MDPI · 2023Article
- DOCK4 Regulation of Rho GTPases Mediates Pulmonary Vascular Barrier Function.Arteriosclerosis, thrombosis, and vascular biology · 2022Article
- Sertoli Cell-Germ Cell Interactions Within the Niche: Paracrine and Juxtacrine Molecular Communications.Frontiers in endocrinology · 2022Review
- Effect of Li-ESWT on Testicular Tissue and Function in Androgen-Deficient Rat Model.Oxidative medicine and cellular longevity · 2022Article
- Testicular torsion: its effect on autoimmunisation, pituitary-testis axis and correlation with primary gonadal dysfunction in boys.Pediatric research · 2021Article
- The "Hitchhiker's Guide to the Galaxy" of Endothelial Dysfunction Markers in Human Fertility.International journal of molecular sciences · 2021Review
- WTAP Function in Sertoli Cells Is Essential for Sustaining the Spermatogonial Stem Cell Niche.Stem cell reports · 2020Article
- Review
- The relationship between vascular endothelial growth factor and spermatogenesis disturbance in an experimentally-induced unilateral cryptorchidism murine model.Molecular biology reports · 2020Article
- Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis.Cell death & disease · 2019Review
- Biology and manipulation technologies of male germline stem cells in mammals.Reproductive medicine and biology · 2018Review
- Expression dynamics of self-renewal factors for spermatogonial stem cells in the mouse testis.The Journal of reproduction and development · 2018Article
- Role of the testis interstitial compartment in spermatogonial stem cell function.Reproduction (Cambridge, England) · 2017Review
- Review
- AKAP9, a Regulator of Microtubule Dynamics, Contributes to Blood-Testis Barrier Function.The American journal of pathology · 2016Article
- VEGFA splicing: divergent isoforms regulate spermatogonial stem cell maintenance.Cell and tissue research · 2016Review
- Vascular endothelial growth factor A: just one of multiple mechanisms for sex-specific vascular development within the testis?The Journal of endocrinology · 2015Review
- Article
- De novo morphogenesis of testis tissue: an improved bioassay to investigate the role of VEGF165 during testis formation.Reproduction (Cambridge, England) · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Vascular endothelial growth factor A (VEGFA) isoform treatment has been demonstrated to alter spermatogonial stem cell homeostasis. Therefore, we generated pDmrt1-Cre;Vegfa(-/-) (knockout, KO) mice by crossing pDmrt1-Cre mice to floxed Vegfa mice to test whether loss of all VEGFA isoforms in Sertoli and germ cells would impair spermatogenesis. When first mated, KO males took 14 days longer to get control females pregnant (P < .02) and tended to take longer for all subsequent parturition intervals (9 days; P < .07). Heterozygous males sired fewer pups per litter (P < .03) and after the first litter took 10 days longer (P < .05) to impregnate females, suggesting a more progressive loss of fertility. Reproductive organs were collected from 6-month-old male mice. There were fewer sperm per tubule in the corpus epididymides (P < .001) and fewer ZBTB16-stained undifferentiated spermatogonia (P < .003) in the testes of KO males. Testicular mRNA abundance for Bcl2 (P < .02), Bcl2:Bax (P < .02), Neurog3 (P < .007), and Ret was greater (P = .0005), tended to be greater for Sin3a and tended to be reduced for total Foxo1 (P < .07) in KO males. Immunofluorescence for CD31 and VE-Cadherin showed no differences in testis vasculature; however, CD31-positive staining was evident in undifferentiated spermatogonia only in KO testes. Therefore, loss of VEGFA isoforms in Sertoli and germ cells alters genes necessary for long-term maintenance of undifferentiated spermatogonia, ultimately reducing sperm numbers and resulting in subfertility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.