ArticlePloS one2013
Transient Inhibition of FGFR2b-ligands signaling leads to irreversible loss of cellular β-catenin organization and signaling in AER during mouse limb development.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Early and transient requirements for FGFR2b/1b ligands in cochlear sensory and neural cell subtype differentiation.Developmental biology · 2025Article
- Fibroblast growth factor 8: Multifaceted role in development and developmental disorder.Genes & diseases · 2025Review
- Germline variants of Brazilian women with breast cancer and detection of a novel pathogenic ATM deletion in early-onset breast cancer.Breast cancer (Tokyo, Japan) · 2021Article
- Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ.Frontiers in cell and developmental biology · 2021Article
- Validation of a NovelFrontiers in cell and developmental biology · 2021Article
- Phosphorylation of Msx1 promotes cell proliferation through the Fgf9/18-MAPK signaling pathway during embryonic limb development.Nucleic acids research · 2020Article
- Evidence for lung repair and regeneration in humans: key stem cells and therapeutic functions of fibroblast growth factors.Frontiers of medicine · 2020Review
- Article
- Fgf10/Fgfr2b Signaling in Mammary Gland Development, Homeostasis, and Cancer.Frontiers in cell and developmental biology · 2020Review
- Fgf10 Signaling-Based Evidence for the Existence of an Embryonic Stage Distinct From the Pseudoglandular Stage During Mouse Lung Development.Frontiers in cell and developmental biology · 2020Article
- Characterization ofFrontiers in genetics · 2019Article
- Spatial and temporal inhibition of FGFR2b ligands reveals continuous requirements and novel targets in mouse inner ear morphogenesis.Development (Cambridge, England) · 2018Article
- A Comprehensive Analysis of Fibroblast Growth Factor Receptor 2b Signaling on Epithelial Tip Progenitor Cells During Early Mouse Lung Branching Morphogenesis.Frontiers in genetics · 2018Article
- Fibroblast Growth Factor 10 and Vertebrate Limb Development.Frontiers in genetics · 2018Review
- Ectoderm-mesoderm crosstalk in the embryonic limb: The role of fibroblast growth factor signaling.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Review
- Fgf10 deficiency is causative for lethality in a mouse model of bronchopulmonary dysplasia.The Journal of pathology · 2017Article
- Rac1 modulates mammalian lung branching morphogenesis in part through canonical Wnt signaling.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Attenuating endogenous Fgfr2b ligands during bleomycin-induced lung fibrosis does not compromise murine lung repair.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Apical ectodermal ridge regulates three principal axes of the developing limb.Journal of Zhejiang University. Science. BReview
- The Fibroblast Growth Factor signaling pathway.Wiley interdisciplinary reviews. Developmental biologyReview
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Authors and funding
13 authors at 7 institutions in 4 countries.
Funding
Abstract
The vertebrate limbs develop through coordinated series of inductive, growth and patterning events. Fibroblast Growth Factor receptor 2b (FGFR2b) signaling controls the induction of the Apical Ectodermal Ridge (AER) but its putative roles in limb outgrowth and patterning, as well as in AER morphology and cell behavior have remained unclear. We have investigated these roles through graded and reversible expression of soluble dominant-negative FGFR2b molecules at various times during mouse limb development, using a doxycycline/transactivator/tet(O)-responsive system. Transient attenuation (≤ 24 hours) of FGFR2b-ligands signaling at E8.5, prior to limb bud induction, leads mostly to the loss or truncation of proximal skeletal elements with less severe impact on distal elements. Attenuation from E9.5 onwards, however, has an irreversible effect on the stability of the AER, resulting in a progressive loss of distal limb skeletal elements. The primary consequences of FGFR2b-ligands attenuation is a transient loss of cell adhesion and down-regulation of P63, β1-integrin and E-cadherin, and a permanent loss of cellular β-catenin organization and WNT signaling within the AER. Combined, these effects lead to the progressive transformation of the AER cells from pluristratified to squamous epithelial-like cells within 24 hours of doxycycline administration. These findings show that FGFR2b-ligands signaling has critical stage-specific roles in maintaining the AER during limb development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.