Evidence map›Paper›PMID 2416734›Full record

ArticleIn vitro cellular & developmental biology : journal of the Tissue Culture Association1985

Intermediate filament expression and lifespan potential in human somatic cell hybrids.

C L Bunn, F A White, W M O'Guin, R H Sawyer, L W Knapp

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology : journal of the Tissue Culture Association, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

C L Bunn
F A White
W M O'Guin
R H Sawyer
L W Knapp

Funding

GENETICS AND BIOCHEMISTRY OF AGING IN HUMAN CELLSR01AG002664 · NIA · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI BUNN, CLIVE L · 1985 to 1985
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MORPHOLOGICAL AND BIOCHEMICAL DEFECTS IN DEVELOPMENTR01HD018129 · NICHD · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI SAWYER, ROGER H · 1985 to 1990
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NIA NIH HHS AG 02664NICHD NIH HHS 1R01 HD 18129-01
6 · The paper itself

Abstract

Limited lifespan human diploid fibroblast cells have been fused with the HeLa derived cell line HEB 7A which possesses transformed growth characteristics and unlimited division potential. HEB 7A expresses keratin intermediate filaments, while the fibroblast cells express only vimentin intermediate filaments. Independently arising clones of hybrids were examined for the presence of keratin by indirect immunofluorescence. Of 11 limited lifespan hybrids, all were keratin negative and possessed the growth characteristics of the fibroblast parent. Of 8 transformed hybrids, 6 arising early after fusion and 2 arising late, all were keratin-positive and simultaneously expressed the transformed growth characteristics of loss of density dependent growth inhibition, low serum dependence, and anchorage independence. It is concluded that the growth properties of these hybrids are associated with the type of intermediate filament expressed. The intermediate filament expression is therefore a marker of proliferative potential in these hybrids.

Indexed as

Cell DivisionCytoskeletonFibroblastsGene Expression RegulationHeLa CellsHumansHybrid CellsIntermediate FilamentsKeratinsTime FactorsKeratins

Identifiers

PMID2416734

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.