ArticleProceedings of the National Academy of Sciences of the United States of America2013
Differential expression of olfactory genes in the southern house mosquito and insights into unique odorant receptor gene isoforms.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed, 126 citations in OpenAlex.
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- Genome-wide identification and expression profiling of odorant receptor genes in the malaria vector Anopheles sinensis.Parasites & vectors · 2022Article
- Human attractive cues and mosquito host-seeking behavior.Trends in parasitology · 2022Review
- Screening of olfactory genes related to blood-feeding behaviors in Culex pipiens quinquefasciatus and Culex pipiens molestus by transcriptome analysis.PLoS neglected tropical diseases · 2022Article
- Article
- Olfactory systems across mosquito species.Cell and tissue research · 2021Review
- Global evaluation of taxonomic relationships and admixture within the Culex pipiens complex of mosquitoes.Parasites & vectors · 2020Article
- Sex- and tissue-specific transcriptome analyses and expression profiling of olfactory-related genes in Ceracris nigricornis Walker (Orthoptera: Acrididae).BMC genomics · 2019Article
- Sequence-Based Prediction of Olfactory Receptor Responses.Chemical senses · 2019Article
- Odorant receptors from Culex quinquefasciatus and Aedes aegypti sensitive to floral compounds.Insect biochemistry and molecular biology · 2019Article
- DEET and other repellents are inhibitors of mosquito odorant receptors for oviposition attractants.Insect biochemistry and molecular biology · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The southern house mosquito, Culex quinquefasciatus, has one of the most acute and eclectic olfactory systems of all mosquito species hitherto studied. Here, we used Illumina sequencing to identify olfactory genes expressed predominantly in antenna, mosquito's main olfactory organ. Less than 50% of the trimmed reads generated by high-quality libraries aligned to a transcript, but approximately 70% of them aligned to the genome. Differential expression analysis, which was validated by quantitative real-time PCR on a subset of genes, showed that approximately half of the 48 odorant-binding protein genes were enriched in antennae, with the other half being predominantly expressed in legs. Similar patterns were observed with chemosensory proteins, "plus-C" odorant-binding proteins, and sensory neuron membrane proteins. Transcripts for as many as 43 ionotropic receptors were enriched in female antennae, thus making the ionotropic receptor family the largest of antennae-rich olfactory genes, second only to odorant receptor (OR) genes. As many as 177 OR genes have been identified, including 36 unique transcripts. The unique OR genes differed from previously annotated ORs in internal sequences, splice variants, and extended N or C terminus. One of the previously unknown transcripts was validated by cloning and functional expression. When challenged with a large panel of physiologically relevant compounds, CquiOR95b responded in a dose-dependent manner to ethyl 2-phenylacteate, which was demonstrated to repel Culex mosquitoes, and secondarily to citronellal, a known insect repellent. This transcriptome study led to identification of key molecular components and a repellent for the southern house mosquito.
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