ArticleChembiochem : a European journal of chemical biology2013
Chemoselective immobilization of proteins by microcontact printing and bio-orthogonal click reactions.
Article in Chembiochem : a European journal of chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Site-Specific Surface Modification via Chemoselective Click Reactions on Cyclobutene-Functionalized Monolayers.ChemistryOpen · 2026Article
- Engineering protein prenylation: an emerging tool for selective protein modification.Biochemical Society transactions · 2025Review
- Biochemistry strategies for label-free optical sensor biofunctionalization: advances towards real applicability.Analytical and bioanalytical chemistry · 2022Review
- A Not-So-Ancient Grease History: Click Chemistry and Protein Lipid Modifications.Chemical reviews · 2021Review
- Recent progress in enzymatic protein labelling techniques and their applications.Chemical Society reviews · 2018Review
- Small-Molecule Patterning via Prefunctionalized Alkanethiols.Chemistry of materials : a publication of the American Chemical Society · 2018Article
- Click chemistry for targeted protein ubiquitylation and ubiquitin chain formation.Nature protocols · 2015Article
- Protein prenylation: enzymes, therapeutics, and biotechnology applications.ACS chemical biology · 2015Review
- Reversible switching between nonquenched and quenched states in nanoscale linear arrays of plant light-harvesting antenna complexes.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Solid phase synthesis of functionalised SAM-forming alkanethiol-oligoethyleneglycols.Journal of materials chemistry. B · 2014Article
- HYDROGEL-BASED NANOCOMPOSITES OF THERAPEUTIC PROTEINS FOR TISSUE REPAIR.Current opinion in chemical engineering · 2014Article
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Authors and funding
9 authors.
Funding
Abstract
Herein, a combination of microcontact printing of functionalized alkanethiols and site-specific modification of proteins is utilized to chemoselectively immobilize proteins onto gold surfaces, either by oxime- or copper-catalyzed alkyne-azide click chemistry. Two molecules capable of click reactions were synthesized, an aminooxy-functionalized alkanethiol and an azide-functionalized alkanethiol, and self-assembled monolayer (SAM) formation on gold was confirmed by IR spectroscopy. The alkanethiols were then individually patterned onto gold surfaces by microcontact printing. Site-specifically modified proteins-horse heart myoglobin (HHMb) containing an N-terminal α-oxoamide and a red fluorescent protein (mCherry-CVIA) with a C-terminal alkyne-were immobilized by incubation onto respective stamped functionalized alkanethiol patterns. Pattern formation was confirmed by fluorescence microscopy.
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