ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1996
Preparation of pea (Pisum sativum L.) chromosome and nucleus suspensions from single root tips.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 51 citations in OpenAlex.
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- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- A flow cytometry-based analysis to establish a cell cycle synchronization protocol for Saccharum spp.Scientific reports · 2020Article
- FISHIS: fluorescence in situ hybridization in suspension and chromosome flow sorting made easy.PloS one · 2013Article
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
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- Perturbation of polyamine catabolism can strongly affect root development and xylem differentiation.Plant physiology · 2011Article
- Significant expansion of Vicia pannonica genome size mediated by amplification of a single type of giant retroelement.Genetics · 2006Article
- PIGY, a new plant envelope-class LTR retrotransposon.Molecular genetics and genomics : MGG · 2005Article
- Flow cytogenetics and plant genome mapping.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Review
- Karyotype analysis of four Vicia species using in situ hybridization with repetitive sequences.Annals of botany · 2003Article
- Chromosome sorting and PCR-based physical mapping in pea (Pisum sativum L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2002Article
- Development of flow cytogenetics and physical genome mapping in chickpea (Cicer arietinum L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2002Article
- Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L.).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1999Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high-yield method for the isolation of intact nuclei and chromosomes in suspension from a variable number of pea root tips (1-10) has been developed. This procedure is based on a two-step cell-cycle synchronization of root-tip meristems to obtain a high mitotic index, followed by formaldehyde fixation and mechanical isolation of chromosomes and nuclei by homogenization. In the explant, up to 50% of metaphases were induced through a synchronization of the cell cycle at the G1/S interface with hydroxyurea (1.25 mM), followed, after a 3-h release, by a block in metaphase with amiprophos-methyl (10 μM). The quality and quantity of nuclei and chromosomes were related to the extent of the fixation. Best results were obtained after a 30-min fixation with 2% and 4% formaldehyde for nuclei and chromosomes, respectively. The method described here allowed the isolation of nuclei and chromosomes, even from a single root tip, with a yield of 1×10(5)/root and 1.4×10(5)/root, respectively. Isolated suspensions were suitable for flow cytometric analysis and sorting and PRINS labelling with a rDNA probe.
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Registered trials
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