ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1996
PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5' -anchored simple sequence repeat (SSR) primers.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- Cytogenomic profiling ofJournal of ginseng research · 2026Article
- Construction of a SSR-based genetic map and identification of QTLs for catechins content in tea plant (Camellia sinensis).PloS one · 2014Article
- Status of feral oilseed rape in Europe: its minor role as a GM impurity and its potential as a reservoir of transgene persistence.Environmental science and pollution research international · 2011Article
- Relatedness of Indian flax genotypes (Linum usitatissimum L.): an inter-simple sequence repeat (ISSR) primer assay.Molecular biotechnology · 2010Article
- Molecular variation and fingerprinting of Leucadendron cultivars (Proteaceae) by ISSR markers.Annals of botany · 2005Article
- Inheritance of seed colour and identification of RAPD and AFLP markers linked to the seed colour gene in rapeseed (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- The development of ISSR-derived SCAR markers around the SEASONAL FLOWERING LOCUS (SFL) in Fragaria vesca.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- Sequence tagged microsatellite profiling (STMP): improved isolation of DNA sequence flanking target SSRs.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primers complementary to simple sequence repeats (SSRs) and with variable three-base 'anchors' at their 5' end, were used in PCR analyses to compare pooled DNA samples from various Brassica napus and B. rapa cultivars. Amplification products were resolved on polyacrylamide gels and detected by silver-nitrate staining. The resulting banding patterns were highly repeatable between replicate PCRs. Two of the primers produced polymorphisms at 33 and 23 band positions, respectively, and could each discriminate 16 of the 20 cultivars studied. Combined use of both primers allowed all 20 cultivars to be distinguished. The UPGMA dendrogram, based on the cultivar banding profiles, demonstrated clustering on the basis of winter/spring growth habit, high/low glucosinolate content, and cultivar origin (i.e. the breeder involved). Intracultivar polymorphism was investigated using a minimum of ten individuals for each cultivar and was found to vary considerably between cultivars. It is concluded that anchored SSR-PCR analysis is a highly informative and reproducible method for fingerprinting oilseed rape populations, but that intra-cultivar variation should be investigated before using banding profiles from pooled samples for the identification of individuals.
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Registered trials
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