ArticleThe American journal of tropical medicine and hygiene2013
Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay.
Article in The American journal of tropical medicine and hygiene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 27 citations in OpenAlex.
- Improved Antibody Detection for Canine Leptospirosis: ELISAs Modified Using Local Leptospiral Serovar Isolates from Asymptomatic Dogs.Animals : an open access journal from MDPI · 2024Article
- An Observational Study of Sepsis in Takeo Province Cambodia: An in-depth examination of pathogens causing severe infections.PLoS neglected tropical diseases · 2020Observational
- An Improved Enzyme-Linked Immunoassay for the Detection ofThe American journal of tropical medicine and hygiene · 2018Article
- Nationwide Seroprevalence of Leptospirosis among Young Thai Men, 2007-2008.The American journal of tropical medicine and hygiene · 2017Article
- Recombinant LipL32 Protein Developed Using a Synthetic Gene Detects Leptospira-specific Antibodies in Human Serum Samples.The Malaysian journal of medical sciences : MJMS · 2017Article
- Diagnostic Accuracy of Recombinant Immunoglobulin-like Protein A-Based IgM ELISA for the Early Diagnosis of Leptospirosis in the Philippines.PLoS neglected tropical diseases · 2015Article
- Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of Leptospira-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and 36 sera were from patients with other febrile diseases from Southeast Asia, where leptospirosis is also endemic. Combining the results of immunoglobulin M (IgM) and IgG detection from these three antigens, the overall sensitivity is close to 90% based on the MAT. These results suggest that an ELISA using multiple recombinant antigens may be used as an alternative method for the detection of Leptospira-specific antibodies.
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Registered trials
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