ArticleNucleic acids research2014
Evaluation of TRAP-sequencing technology with a versatile conditional mouse model.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 54 citations in OpenAlex.
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- Key epigenetic and signaling factors in the formation and maintenance of the blood-brain barrier.eLife · 2024Article
- CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
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- Organotypic heterogeneity in microvascular endothelial cell responses in sepsis-a molecular treasure trove and pharmacological Gordian knot.Frontiers in medicine · 2023Review
- Molecular analysis of vascular gene expression.Research and practice in thrombosis and haemostasis · 2022Article
- Spatio-molecular domains identified in the mouse subthalamic nucleus and neighboring glutamatergic and GABAergic brain structures.Communications biology · 2020Article
- The in vivo endothelial cell translatome is highly heterogeneous across vascular beds.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- What Are 3' UTRs Doing?Cold Spring Harbor perspectives in biology · 2019Review
- Disruption of the Extracellular Matrix Progressively Impairs Central Nervous System Vascular Maturation Downstream of β-Catenin Signaling.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- Cell-specific image-guided transcriptomics identifies complex injuries caused by ischemic acute kidney injury in mice.Communications biology · 2019Article
- Arhgef12 drives IL17A-induced airway contractility and airway hyperresponsiveness in mice.JCI insight · 2018Article
- SLAM-ITseq: sequencing cell type-specific transcriptomes without cell sorting.Development (Cambridge, England) · 2018Article
- A comprehensive map coupling histone modifications with gene regulation in adult dopaminergic and serotonergic neurons.Nature communications · 2018Article
- The Expanding Toolkit of Translating Ribosome Affinity Purification.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Hedgehog signaling regulates ciliary localization of mouse odorant receptors.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Analysis of Ribosome-Associated mRNAs in Rice Reveals the Importance of Transcript Size and GC Content in Translation.G3 (Bethesda, Md.) · 2017Article
- Human splicing diversity and the extent of unannotated splice junctions across human RNA-seq samples on the Sequence Read Archive.Genome biology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression profiling of various cell lineages has provided invaluable insights into the molecular mechanisms regulating cellular development and differentiation. However, in vivo molecular profiling of rare and interspersed cell populations, such as endothelial cells, has remained challenging. We have generated a versatile floxed translating ribosome affinity purification (TRAP) mouse model, mCherryTRAP, for Cre-dependent translational profiling of distinct cell lineages from intact tissues. To identify cell type-specific transcripts using TRAP, the data have to be filtered to remove both background transcripts not expressed in the profiled cell type and transcripts expressed in all cell populations of the tissue/organ. Filtering has previously been achieved using transcribed RNA from the tissue/organ. Using the mCherryTRAP model, we demonstrate extensive differential expression of RNAs between the translatome and transcriptome of embryonic brains and kidneys. We evaluate the implications of these data for TRAP studies of abundant and rare cell populations. Finally, we demonstrate the applicability of the technology to study organ-specific endothelial cell differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.