ArticleBMC research notes2013
Vectorial secretion of interleukin-8 mediates autocrine signalling in intestinal epithelial cells via apically located CXCR1.
Article in BMC research notes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Characterization of the inflammatory response of a canine intestinal epithelial cell line challenged with lipopolysaccharides and/or butyrate.Journal of animal science · 2026Article
- Development of an intestinal mucosaJournal of virology · 2024Article
- Systematic comparison of transcriptomes of Caco-2 cells cultured under different cellular and physiological conditions.Archives of toxicology · 2023Article
- The yin and yang of B cells in a constant state of battle: intestinal inflammation and inflammatory bowel disease.Frontiers in immunology · 2023Review
- FoxP3 expression by retinal pigment epithelial cells: transcription factor with potential relevance for the pathology of age-related macular degeneration.Journal of neuroinflammation · 2022Article
- Intestinal secretory mechanisms and diarrhea.American journal of physiology. Gastrointestinal and liver physiology · 2022Review
- Anti-Inflammatory Effect of anInternational journal of molecular sciences · 2022Article
- Review
- Faecal cytokine profiling as a marker of intestinal inflammation in acutely decompensated cirrhosis.JHEP reports : innovation in hepatology · 2020Article
- Effects of undigested protein-rich ingredients on polarised small intestinal organoid monolayers.Journal of animal science and biotechnology · 2020Article
- High-level integration of murine intestinal transcriptomics data highlights the importance of the complement system in mucosal homeostasis.BMC genomics · 2019Article
- Cytokine Tuning of Intestinal Epithelial Function.Frontiers in immunology · 2018Review
- Mechanisms of Cell Polarity-Controlled Epithelial Homeostasis and Immunity in the Intestine.Cold Spring Harbor perspectives in biology · 2017Review
- Tissue-Intrinsic Tumor Hotspots: Terroir for Tumorigenesis.Trends in cancer · 2017Review
- Homeostasis of the gut barrier and potential biomarkers.American journal of physiology. Gastrointestinal and liver physiology · 2017Review
- Can probiotics modulate human disease by impacting intestinal barrier function?The British journal of nutrition · 2017Review
- Epithelial Tumors Originate in Tumor Hotspots, a Tissue-Intrinsic Microenvironment.PLoS biology · 2016Article
- Secretion of Alpha-Hemolysin by Escherichia coli Disrupts Tight Junctions in Ulcerative Colitis Patients.Clinical and translational gastroenterology · 2016Article
- Flagellin Induces β-Defensin 2 in Human Colonic Ex vivo Infection with Enterohemorrhagic Escherichia coli.Frontiers in cellular and infection microbiology · 2016Article
- Cellular Barriers after Extravasation: Leukocyte Interactions with Polarized Epithelia in the Inflamed Tissue.Mediators of inflammation · 2016Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the intestinal mucosa, several adaptations of TLR signalling have evolved to avoid chronic inflammatory responses to the presence of commensal microbes. Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus.
resultsIn the Caco-2 BBE model of polarized villus-like epithelium, apical stimulation with TLR2 and TLR5 ligands resulted in the apical secretion of IL-8. The CXCR1 receptor for IL-8 was expressed only on the apical membrane of Caco-2 BBE cells and differentiated epithelial cells in the human small intestine and colon. Transcriptome analyses revealed that Caco-2 BBE cells respond to stimulation with IL-8 supporting the hypothesis that IL-8 induces G protein-coupled receptor signalling.
conclusionsThese results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo, suggesting an autocrine function for IL-8 secreted in the lumen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.