ArticleMolecular microbiology2013
Complex formation and processing of the minor transformation pilins of Bacillus subtilis.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Molecular mechanisms and applications of natural transformation in bacteria.Frontiers in microbiology · 2025Review
- The ins and outs of Bacillus proteases: activities, functions and commercial significance.FEMS microbiology reviews · 2022Review
- Article
- The Role of Minor Pilins in Assembly and Function of the Competence Pilus ofFrontiers in cellular and infection microbiology · 2021Article
- Unleashing Natural Competence in Lactococcus lactis by Induction of the Competence Regulator ComX.Applied and environmental microbiology · 2017Article
- Determining the Composition and Stability of Protein Complexes Using an Integrated Label-Free and Stable Isotope Labeling Strategy.Methods in molecular biology (Clifton, N.J.) · 2016Article
- In vivo characterization of the scaffold activity of flotillin on the membrane kinase KinC of Bacillus subtilis.Microbiology (Reading, England) · 2015Article
- ComGA-RelA interaction and persistence in the Bacillus subtilis K-state.Molecular microbiology · 2015Article
- Conserved Streptococcus pneumoniae spirosomes suggest a single type of transformation pilus in competence.PLoS pathogens · 2015Article
- Uptake of extracellular DNA: competence induced pili in natural transformation of Streptococcus pneumoniae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Exploring the existence of lipid rafts in bacteria.Microbiology and molecular biology reviews : MMBR · 2015Article
- Bacterial transformation: distribution, shared mechanisms and divergent control.Nature reviews. Microbiology · 2014Review
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Transformation in most bacteria is dependent on orthologues of Type 2 secretion and Type 4 pilus system proteins. In each system, pilin proteins (major and minor) are required to make the pilus structure and are essential to the process, although the precise roles of the minor pilins remain unclear. We have explored protein-protein interactions among the competence minor pilins of Bacillus subtilis through in vitro binding studies, immunopurification and mass spectrometry. We demonstrate that the minor pilins directly interact, and the minor pilin ComGG interacts with most of the known proteins required for transformation. We find that ComGG requires other ComG proteins for its stabilization and for processing by the pre-pilin peptidase. These observations, C-terminal mutations in ComGG that prevent processing and the inaccessibility of pre-ComGG to externally added protease suggest a model in which pre-ComGG must be associated with other minor pilins for processing to take place. We propose that ComGG does not become a transmembrane protein until after processing. These behaviours contrast with that of pre-ComGC, the major pilin, which is accessible to externally added protease and requires only the peptidase to be processed. The roles of the pilins and of the pilus in transformation are discussed.
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Registered trials
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