ArticleJournal of the American Chemical Society2013
Electrochemical assay for the signal-on detection of human DNA methyltransferase activity.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 113 citations in OpenAlex.
- Microelectrode Arrays, Electrocatalysis, and the Need for Proper Characterization.ChemElectroChem · 2023Article
- Molecular wrench activity of DNA helicases: Keys to modulation of rapid kinetics in DNA repair.Protein science : a publication of the Protein Society · 2023Article
- Controlling Dynamic DNA Reactions at the Surface of Single-Walled Carbon Nanotube Electrodes to Design Hybridization Platforms with a Specific Amperometric Readout.Analytical chemistry · 2022Article
- DNA Electrochemistry: Charge-Transport Pathways through DNA Films on Gold.Journal of the American Chemical Society · 2021Article
- Ultrasensitive DNA methyltransferase activity sensing and inhibitor evaluation with highly photostable upconversion nanoparticle transducer.Mikrochimica acta · 2021Article
- Solidly mounted resonator sensor for biomolecule detections.RSC advances · 2019Article
- Electrochemical determination of the activity of DNA methyltransferase based on the methyl binding domain protein and a customized modular detector.Mikrochimica acta · 2019Article
- Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample.International journal of nanomedicine · 2018Article
- Electrical Probes of DNA-Binding Proteins.Methods in enzymology · 2017Article
- Helix-Dependent Spin Filtering through the DNA Duplex.Journal of the American Chemical Society · 2016Article
- Redox Signaling through DNA.Israel journal of chemistry · 2016Article
- A MoS₂ Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation.Sensors (Basel, Switzerland) · 2016Article
- DNA Charge Transport: from Chemical Principles to the Cell.Cell chemical biology · 2016Review
- DNA Methyltransferase Activity Assays: Advances and Challenges.Theranostics · 2016Review
- DNA Electrochemistry Shows DNMT1 Methyltransferase Hyperactivity in Colorectal Tumors.Chemistry & biology · 2015Article
- Label-free electrochemical detection of human methyltransferase from tumors.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Strategies to detect human DNA methyltransferases are needed, given that aberrant methylation by these enzymes is associated with cancer initiation and progression. Here we describe a nonradioactive, antibody-free, electrochemical assay in which methyltransferase activity on DNA-modified electrodes confers protection from restriction for signal-on detection. We implement this assay with a multiplexed chip platform and show robust detection of both bacterial (SssI) and human (Dnmt1) methyltransferase activity. Essential to work with human methyltransferases, our unique assay design allows activity measurements on both unmethylated and hemimethylated DNA substrates. We validate this assay by comparison with a conventional radioactive method. The advantages of electrochemistry over radioactivity and fluorescence make this assay an accessible and promising new approach for the sensitive, label-free detection of human methyltransferase activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.