ArticleHuman molecular genetics2014
Cross-sectional and longitudinal changes in DNA methylation with age: an epigenome-wide analysis revealing over 60 novel age-associated CpG sites.
Article in Human molecular genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 177 papers, 4 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
177 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Pooled it
- Advances in body fluid identification: MiRNA markers as powerful tool.International journal of legal medicine · 2024Pooled it
- Systematic review supports the role of DNA methylation in the pathophysiology of preeclampsia: a call for analytical and methodological standardization.Biology of sex differences · 2020Pooled it
- Pooled it
- Prospective study of DNA methylation at LINE-1 and Alu in peripheral blood and the risk of prostate cancer.The Prostate · 2015Trial
- DNA Methylation Predicts Progression of Human Gastric Lesions.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2015Trial
- Development of a precise saliva-based epigenetic clock using a six-CpG-marker panel.International journal of legal medicine · 2026Article
- Chronological age estimation using CpG methylation signatures in Pakistani population.International journal of legal medicine · 2026Article
- Low-Pass Nanopore Sequencing of Plasma cfDNA Reveals Fragmentomic, Epigenomic, and Age-Associated Signatures Under Ultra-Low-Coverage Conditions.International journal of molecular sciences · 2026Article
- Article
- Less-invasive age estimation using hair based on DNA methylation in brown bears.Scientific reports · 2025Article
- Postnatal epigenetic differences in calves following transient fetal infection with bovine viral diarrhea virus.BMC genomics · 2025Article
- Plasma Proteomic Signature as a Predictor of Age Advancement in People Living With HIV.Aging cell · 2025Article
- A methylation panel of 10 CpGs for accurate age inference via stepwise conditional epigenome-wide association study.International journal of legal medicine · 2025Article
- Epigenetic Clock in Bears: A Simple Cost-Effective Blood DNA Methylation-Based Age Estimation Method Applicable to Multiple Bear Species.Ecology and evolution · 2025Article
- Weighted 2D-kernel density estimations provide a new probabilistic measure for epigenetic age.Genome biology · 2025Article
- The Effectiveness of a Race-Based Stress Reduction Intervention on Improving Stress-Related Symptoms and Inflammation in African American Women at Risk for Cardiometabolic Disease: Protocol for Recruitment and Intervention for a Randomized Controlled Trial.JMIR research protocols · 2025Article
- Epigenetic biomarkers of socioeconomic status are associated with age-related chronic diseases and mortality in older adults.PNAS nexus · 2025Article
- Age-related changes in DNA methylation in a sample of elderly Brazilians.Clinical epigenetics · 2025Article
- The Long Noncoding RNA PANDAR as a Prognostic and Diagnostic Biomarker in Panceratic Cancer.Current cancer drug targets · 2025Review
117 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the role of epigenetic modifications, e.g. DNA methylation, in the process of aging requires the characterization of methylation patterns in large cohorts. We analysed >480 000 CpG sites using Infinium HumanMethylation450 BeadChip (Illumina) in whole blood DNA of 965 participants of a population-based cohort study aged between 50 and 75 years. In an exploratory analysis in 400 individuals, 200 CpG sites with the highest Spearman correlation coefficients for the association between methylation and age were identified. Of these 200 CpGs, 162 were significantly associated with age, which was verified in an independent cohort of 498 individuals using mixed linear regression models adjusted for gender, smoking behaviour, age-related diseases and random batch effect and corrected for multiple testing by Bonferroni. In another independent cohort of 67 individuals without history of major age-related diseases and with a follow-up of 8 years, we observed a gain in methylation at 96% (52%, significant) of the positively age-associated CpGs and a loss at all (89%, significant) of the negatively age-associated CpGs in each individual while getting 8 years older. A regression model for age prediction based on 17 CpGs as predicting variables explained 71% of the variance in age with an average accuracy of 2.6 years. In comparison with cord blood samples obtained from the Ulm Birth Cohort Study, we observed a more than 2-fold change in mean methylation levels from birth to older age at 86 CpGs. We were able to identify 65 novel CpG sites with significant association of methylation with age.
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