ArticleApplied and environmental microbiology2014
Monitoring rates and heterogeneity of high-pressure germination of bacillus spores by phase-contrast microscopy of individual spores.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- Article
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
- High-pressure continuous culturing: life at the extreme.Applied and environmental microbiology · 2025Review
- Strategies for effective high pressure germination or inactivation ofApplied and environmental microbiology · 2024Article
- Identification and characterization of new proteins crucial for bacterial spore resistance and germination.Frontiers in microbiology · 2023Article
- Facilitated endospore detection forEngineering in life sciences · 2022Article
- Moderate High-Pressure Superdormancy inApplied and environmental microbiology · 2022Article
- Flow Cytometry Combined With Single Cell Sorting to Study Heterogeneous Germination ofFrontiers in microbiology · 2019Article
- Building of Pressure-Assisted Ultra-High Temperature System and Its Inactivation of Bacterial Spores.Frontiers in microbiology · 2019Article
- Effects of High Pressure on Bacillus licheniformis Spore Germination and Inactivation.Applied and environmental microbiology · 2017Article
- Activation and inactivation of Bacillus pumilus spores by kiloelectron volt X-ray irradiation.PloS one · 2017Article
- Use of Raman Spectroscopy and Phase-Contrast Microscopy To Characterize Cold Atmospheric Plasma Inactivation of Individual Bacterial Spores.Applied and environmental microbiology · 2016Article
- Effects of High-Pressure Treatment on Spores of Clostridium Species.Applied and environmental microbiology · 2016Article
- Article
- Slow leakage of Ca-dipicolinic acid from individual bacillus spores during initiation of spore germination.Journal of bacteriology · 2015Article
- High pressure thermal inactivation of Clostridium botulinum type E endospores - kinetic modeling and mechanistic insights.Frontiers in microbiology · 2015Article
- Fighting Ebola with novel spore decontamination technologies for the military.Frontiers in microbiology · 2015Article
- High-precision fitting measurements of the kinetics of size changes during germination of individual Bacillus spores.Applied and environmental microbiology · 2014Article
- Monitoring of commitment, blocking, and continuation of nutrient germination of individual Bacillus subtilis spores.Journal of bacteriology · 2014Article
- Analysis of the loss in heat and acid resistance during germination of spores of Bacillus species.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germination of Bacillus spores with a high pressure (HP) of ∼150 MPa is via activation of spores' germinant receptors (GRs). The HP germination of multiple individual Bacillus subtilis spores in a diamond anvil cell (DAC) was monitored with phase-contrast microscopy. Major conclusions were that (i) >95% of wild-type spores germinated in 40 min in a DAC at ∼150 MPa and 37°C but individual spores' germination kinetics were heterogeneous; (ii) individual spores' HP germination kinetic parameters were similar to those of nutrient-triggered germination with a variable lag time (Tlag) prior to a period of the rapid release (ΔTrelease) of the spores' dipicolinic acid in a 1:1 chelate with Ca(2+) (CaDPA); (iii) spore germination at 50 MPa had longer average Tlag values than that at ∼150 MPa, but the ΔTrelease values at the two pressures were identical and HPs of <10 MPa did not induce germination; (iv) B. subtilis spores that lacked the cortex-lytic enzyme CwlJ and that were germinated with an HP of 150 MPa exhibited average ΔTrelease values ∼15-fold longer than those for wild-type spores, but the two types of spores exhibited similar average Tlag values; and (v) the germination of wild-type spores given a ≥30-s 140-MPa HP pulse followed by a constant pressure of 1 MPa was the same as that of spores exposed to a constant pressure of 140 MPa that was continued for ≥35 min; (vi) however, after short 150-MPa HP pulses and incubation at 0.1 MPa (ambient pressure), spore germination stopped 5 to 10 min after the HP was released. These results suggest that an HP of ∼150 MPa for ≤30 s is sufficient to fully activate spores' GRs, which remain activated at 1 MPa but can deactivate at ambient pressure.
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