ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1996
Comparative analysis of genetic diversity in pea assessed by RFLP- and PCR-based methods.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed.
- Phenotypic diversity and provenance variation ofPeerJ · 2024Article
- Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (Plants (Basel, Switzerland) · 2022Article
- Genetic diversity and population structure among pea (Pisum sativum L.) cultivars as revealed by simple sequence repeat and novel genic markers.Molecular biotechnology · 2014Article
- Amplified fragment length polymorphism: an adept technique for genome mapping, genetic differentiation, and intraspecific variation in protozoan parasites.Parasitology research · 2013Review
- Analysis of a diverse global Pisum sp. collection and comparison to a Chinese local P. sativum collection with microsatellite markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Comparative analyses of genetic diversities within tomato and pepper collections detected by retrotransposon-based SSAP, AFLP and SSR.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Genetic diversity within Pisum sativum using protein- and PCR-based markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- Genetic relationships among perennial and annual Cicer species growing in Turkey assessed by AFLP fingerprinting.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- AFLP analysis of genetic variability in New Guinea impatiens.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003Article
- Amplified-fragment length polymorphism analysis: the state of an art.Journal of clinical microbiology · 1999Review
- AFLPs represent highly repetitive sequences in Asparagus officinalis L.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1999Article
- Assessing genetic diversity in a sugarcane germplasm collection using an automated AFLP analysis.Genetica · 1998Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA-based molecular-marker techniques have been proven powerful in genetic diversity estimations. Among them, RFLP was the first and is still the most commonly used in the estimation of genetic diversity of eukaryotic species. The recently developed PCR-based multiple-loci marker techniques, which include RAPD, AFLP, Microsatellite-AFLP and inter-SSR PCR, are playing increasingly important roles in this type of research. Despite the wide application of these techniques, no direct comparison of these methods in the estimation of genetic diversity has been carried out. Here we report a direct comparison of DNA-based RFLP with various PCR-based techniques regarding their informativeness and applicability for genetic diversity analysis. Among ten pea genotypes studied, all the PCR-based methods were much more informative than cDNA-RFLP. Genetic diversity trees were derived from each marker technique, and compared using Mantel's test. By this criterion, all trees derived from the various molecular marker techniques, except for the tree derived from inter-SSR PCR, were significantly correlated, suggesting that these PCR-based techniques could replace RFLP in the estimation of genetic diversity. On the basis of this result, AFLP analysis was applied to assess the genetic diversity of a sample of accessions representing the various species and subspecies within the genus Pisum.
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Registered trials
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