ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1996
Allele-specific amplification of polymorphic sites for the detection of powdery mildew resistance loci in cereals.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Identifying a novel powdery mildew resistance gene in a barley landrace from Morocco.Journal of applied genetics · 2019Article
- Molecular mapping of a new powdery mildew resistance gene Pm2b in Chinese breeding line KM2939.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- CAPS markers improved by cluster-specific amplification for identification of octoploid strawberry (Fragaria x ananassa Duch.) cultivars, and their disomic inheritance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Sequence haplotypes revealed by sequence-tagged site fine mapping of the Ror1 gene in the centromeric region of barley chromosome 1H.Plant physiology · 2001Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primers for the polymerase chain reaction (PCR) were tailored to selectively amplify RFLP marker alleles associated with resistance and susceptibility for powdery mildew in cereals. The differentiation between marker alleles for susceptible and resistant genotypes is based on the discrimination of a single nucleotide by using allele-specific oligonucleotides as PCR primers. The PCR assays developed are diagnostic for RFLP alleles at the loci MWG097 in the barley genome and Whs350 in the wheat genome. The first marker locus is closely linked to MlLa resistance in barley, while the latter is linked to Pm2 resistance locus in wheat. PCR analysis of 31 barley and 30 wheat cultivars, with some exceptions, verified the presence or absence of the resistance loci investigated. These rapid PCR-based approaches are proposed as an efficient alternative to conventional procedures for selecting powdery mildew-resistant genotypes in breeding programs.
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Registered trials
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