ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1996
Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
29 citing papers in PubMed.
- Development of genome-specific SSR markers for the identification of introgressed segments of3 Biotech · 2022Article
- The first genetic map of a synthesized allohexaploid Brassica with A, B and C genomes based on simple sequence repeat markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Analysis of population genetic structure and gene flow in an annual plant before and after a rapid evolutionary response to drought.AoB PLANTS · 2015Article
- A combined linkage and regional association mapping validation and fine mapping of two major pleiotropic QTLs for seed weight and silique length in rapeseed (Brassica napus L.).BMC plant biology · 2014Article
- Transcriptome sequencing of two parental lines of cabbage (Brassica oleracea L. var. capitata L.) and construction of an EST-based genetic map.BMC genomics · 2014Article
- Genome-wide microsatellite characterization and marker development in the sequenced Brassica crop species.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Article
- Identification of putative quantitative trait loci associated with a flavonoid related to resistance to cabbage seedpod weevil (Ceutorhynchus obstrictus) in canola derived from an intergeneric cross, Sinapis alba × Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Seed coat microsculpturing is related to genomic components in wild Brassica juncea and Sinapis arvensis.PloS one · 2013Article
- Identification of a major QTL for silique length and seed weight in oilseed rape (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- A new broccoli × broccoli immortal mapping population and framework genetic map: tools for breeders and complex trait analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Development of SSR markers and construction of a linkage map in jute.Journal of genetics · 2012Article
- Article
- Relationships among the A Genomes of Triticum L. species as evidenced by SSR markers, in Iran.International journal of molecular sciences · 2010Article
- Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa.BMC genomics · 2010Article
- Molecular differentiation of commercial varieties and feral populations of oilseed rape (Brassica napus L.).BMC evolutionary biology · 2010Article
- Development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling Brassica rapa and B. oleracea.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Minimising inter-laboratory variation when constructing a unified molecular database of plant varieties in an allogamous crop.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Development of a set of public SSR markers derived from genomic sequence of a rapid cycling Brassica oleracea L. genotype.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of simple sequence repeat (SSR) genotyping for the characterization of genetic variation in crop plants has been hindered by ready access to useful primer pairs and potentially limited conservation of the repeat sequences among related species. In this phase of work, we report on the identification and characterization of SSRs that are conserved in Brassica napus L. (rapeseed) and its putative progenitors, B. oleracea L. (cabbage, and related vegetable types) and B. rapa (vegetable and oil types). Approximately 140 clones from a size-fractionated genomic library of B. napus were sequenced, and primer pairs were designed for 21 dinucleotide SSRs. Seventeen primer pairs amplified products in the three species and, among these, 13 detected variation between and within species. Unlike findings on SSR information content in human, no relationship could be established between the number of tandem repeats within the target sequence and heterozygosity. All primer pairs have been designed to work under identical amplification conditions; therefore, single-reaction, multiplex polymerase chain reaction (PCR) with these SSRs is possible. Once moderate numbers of primer pairs are accessible to the user community, SSR genotyping may provide a useful method for the characterization, conservation, and utilization of agricultural crop diversity.
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Registered trials
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