ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1996
Introduction of a gene from fertility restored radish (Raphanus sativus) into Brassica napus by fusion of X-irradiated protoplasts from a radish restorer line and iodacetoamide-treated protoplasts from a cytoplasmic male-sterile cybrid of B. napus.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 47 citations in OpenAlex.
- Exploring the gene pool of Brassica napus by genomics-based approaches.Plant biotechnology journal · 2021Review
- Genetic characterization of a new radish introgression line carrying the restorer gene for Ogura CMS in Brassica napus.PloS one · 2020Article
- Cytoplasmic male sterility in Brassicaceae crops.Breeding science · 2014Review
- Construction of a chromosome-assigned, sequence-tagged linkage map for the radish, Raphanus sativus L. and QTL analysis of morphological traits.Breeding science · 2013Article
- Development of a novel Sinapis arvensis disomic addition line in Brassica napus containing the restorer gene for Nsa CMS and improved resistance to Sclerotinia sclerotiorum and pod shattering.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- QTL mapping of clubroot resistance in radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Identification of mitochondrial genome rearrangements unique to novel cytoplasmic male sterility in radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Discovery of a novel cytoplasmic male-sterility and its restorer lines in radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- A new recombined double low restorer line for the Ogu-INRA cms in rapeseed (Brassica napus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Somatic hybrids between Arabidopsis thaliana and cytoplasmic male-sterile radish (Raphanus sativus).Plant cell reports · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To establish a cytoplasmic male-sterile/restored fertility (cms-Rf) system for F1 seed production in Brassica napus, we transferred a gene from fertillity restored radish to B. napus by protoplast fusion. X-irradiated protoplasts, isolated from shoots of Raphanus sativus cv Kosena (Rf line), were fused with iodoacetamide-treated protoplasts of a B. napus cms cybrid. Among 300 regenerated plants, six were male-fertile. The fertile plants were characterized for petal color, chromosome number and the percentage of viable pollen grains. Three fertile plants had aneuploid chromosome numbers and white or cream petals, which is a dominant marker in radish. Of these three plants, one which had 2n = 47 chromosomes and white petals was used for further backcrosses. After two backcrosses, chromosome number and petal color became identical to that of B. napus. No female sterility was observed in the BC3 generations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.