ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1996
Multiple methods for the identification of polymorphic simple sequence repeats (SSRs) in sorghum [Sorghum bicolor (L.) Moench].
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 293 citations in OpenAlex.
- Dissecting genetic diversity and population structure in intergeneric citrus population using morphological and SSR markers.Scientific reports · 2026Article
- Genetic diversity and population structure of Ethiopian Sweet Sorghum [Sorghum bicolor (L.) Moench] germplasms using SSR markers.PloS one · 2025Article
- Article
- De novo identification and targeted sequencing of SSRs efficiently fingerprints Sorghum bicolor sub-population identity.PloS one · 2021Article
- The genetic relationships of Indian jujube (Heliyon · 2020Article
- Genetic diversity and population structure of the endangered orchid Pelatantheria scolopendrifolia (Orchidaceae) in Korea.PloS one · 2020Article
- Induction of apomixis by dimethyl sulfoxide (DMSO) and genetic identification of apomictic plants in cassava.Breeding science · 2018Article
- Molecular mapping and candidate gene analysis of a new epicuticular wax locus in sorghum (Sorghum bicolor L. Moench).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- GBS-based single dosage markers for linkage and QTL mapping allow gene mining for yield-related traits in sugarcane.BMC genomics · 2017Article
- Introgression of Shoot Fly (Frontiers in plant science · 2017Article
- Phylogenetic and Diversity Analysis of Dactylis glomerata Subspecies Using SSR and IT-ISJ Markers.Molecules (Basel, Switzerland) · 2016Article
- Sorghum Dw1, an agronomically important gene for lodging resistance, encodes a novel protein involved in cell proliferation.Scientific reports · 2016Article
- Analysis of in situ diversity and population structure in Ethiopian cultivated Sorghum bicolor (L.) landraces using phenotypic traits and SSR markers.SpringerPlus · 2014Article
- Assessment of genetic diversity in the sorghum reference set using EST-SSR markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Estimation of in situ mating systems in wild sorghum (Sorghum bicolor (L.) Moench) in Ethiopia using SSR-based progeny array data: implications for the spread of crop genes into the wild.Journal of genetics · 2013Article
- Genetic analysis of recombinant inbred lines for Sorghum bicolor × Sorghum propinquum.G3 (Bethesda, Md.) · 2013Article
- Studying the genetic basis of drought tolerance in sorghum by managed stress trials and adjustments for phenological and plant height differences.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Genetic structure and diversity of wild sorghum populations (Sorghum spp.) from different eco-geographical regions of Kenya.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Development and assessment of DArT markers in triticale.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Wild sorghum from different eco-geographic regions of Kenya display a mixed mating system.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simple sequence repeats (SSRs), also known as microsatellites, are highly variable DNA sequences that can be used as markers for the genetic analysis of plants. Three approaches were followed for the development of PCR primers for the amplification of DNA fragments containing SSRs from sorghum [Sorghum bicolor (L.) Moench]: a search for sorghum SSRs in public DNA databases; the use of SSR-specific primers developed in the Poaceae species maize (Zea mays L.) and seashore paspalum grass (Paspalum vaginatum Swartz); and the screening of sorghum genomic libraries by hybridization with SSR oligonucleotides. A total of 49 sorghum SSR-specific PCR primer pairs (two designed from GenBank SSR-containing sequences and 47 from the sequences of genomic clones) were screened on a panel of 17 sorghum and one maize accession. Ten primer pairs from paspalum and 90 from maize were also screened for polymorphism in sorghum. Length polymorphisms among amplification products were detected with 15 of these primer pairs, yielding diversity values ranging from 0.2 to 0.8 with an average diversity of 0.56. These primer pairs are now available for use as markers in crop improvement and conservation efforts.
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