ArticleJournal of neuroscience methods2014
A microchip for quantitative analysis of CNS axon growth under localized biomolecular treatments.
Article in Journal of neuroscience methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 63 citations in OpenAlex.
- In Vitro Reconstruction of Axonal Heat Sensing with a Photothermal Nerve-on-a-Chip.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Nervous System-on-Chip: Innovative Microfluidic Platform to Compartmentalize hiPSC-Derived Neural Networks.Micromachines · 2026Article
- Biomaterials for neuroengineering: applications and challenges.Regenerative biomaterials · 2025Review
- Recent Progress and Perspectives on Neural Chip Platforms Integrating PDMS-Based Microfluidic Devices and Microelectrode Arrays.Micromachines · 2023Review
- Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation.Bioengineering (Basel, Switzerland) · 2022Review
- Stimulus-Evoked Activity Modulation of In Vitro Engineered Cortical and Hippocampal Networks.Micromachines · 2022Article
- Neurons-on-a-Chip:Molecules and cells · 2022Review
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- Optimization of PTFE Coating on PDMS Surfaces for Inhibition of Hydrophobic Molecule Absorption for Increased Optical Detection Sensitivity.Sensors (Basel, Switzerland) · 2021Article
- Application of New Materials in Auditory Disease Treatment.Frontiers in cellular neuroscience · 2021Review
- Recent progress in translational engineered in vitro models of the central nervous system.Brain : a journal of neurology · 2020Review
- Bioprinting Neural Systems to Model Central Nervous System Diseases.Advanced functional materials · 2020Review
- A magnetically actuated microrobot for targeted neural cell delivery and selective connection of neural networks.Science advances · 2020Article
- Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems.Biosensors · 2020Review
- Evolution of Biochip Technology: A Review from Lab-on-a-Chip to Organ-on-a-Chip.Micromachines · 2020Review
- Nanogrooves for 2D and 3D Microenvironments of SH-SY5Y Cultures in Brain-on-Chip Technology.Frontiers in neuroscience · 2020Article
- Microfluidic Brain-on-a-Chip: Perspectives for Mimicking Neural System Disorders.Molecular neurobiology · 2019Review
- An open-type microdevice to improve the quality of fluorescence labeling for axonal transport analysis in neurons.Biomicrofluidics · 2019Article
- Modulation of Neural Activity for Myelination in the Central Nervous System.Frontiers in neuroscience · 2019Review
- The Need for Physiological Micro-Nanofluidic Systems of the Brain.Frontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Growth capability of neurons is an essential factor in axon regeneration. To better understand how microenvironments influence axon growth, methods that allow spatial control of cellular microenvironments and easy quantification of axon growth are critically needed. Here, we present a microchip capable of physically guiding the growth directions of axons while providing physical and fluidic isolation from neuronal somata/dendrites that enables localized biomolecular treatments and linear axon growth. The microchip allows axons to grow in straight lines inside the axon compartments even after the isolation; therefore, significantly facilitating the axon length quantification process. We further developed an image processing algorithm that automatically quantifies axon growth. The effect of localized extracellular matrix components and brain-derived neurotropic factor treatments on axon growth was investigated. Results show that biomolecules may have substantially different effects on axon growth depending on where they act. For example, while chondroitin sulfate proteoglycan causes axon retraction when added to the axons, it promotes axon growth when applied to the somata. The newly developed microchip overcomes limitations of conventional axon growth research methods that lack localized control of biomolecular environments and are often performed at a significantly lower cell density for only a short period of time due to difficulty in monitoring of axonal growth. This microchip may serve as a powerful tool for investigating factors that promote axon growth and regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.