Evidence map›Paper›PMID 24161167›Full record

ArticleBiomaterials2014

The enhancement of bone regeneration by gene activated matrix encoding for platelet derived growth factor.

Satheesh Elangovan, Sheetal R D'Mello, Liu Hong, Ryan D Ross, Chantal Allamargot, Deborah V Dawson, Clark M Stanford, Georgia K Johnson, D Rick Sumner, Aliasger K Salem

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed
15.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 139 citations in OpenAlex.

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  11. Matrices Activated with Messenger RNA.Journal of functional biomaterials · 2023
    Review
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  15. Comparison ofBiofabrication · 2022
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3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Satheesh ElangovanDepartment of Periodontics, University of Iowa College of Dentistry, Iowa City, IA, USA. Electronic address: satheesh-elangovan@uiowa.edu.
Sheetal R D'Mello
Liu Hong
Ryan D Ross
Chantal Allamargot
Deborah V Dawson
Clark M Stanford
Georgia K Johnson
D Rick Sumner
Aliasger K Salem
University of Iowa · USRush University Medical Center · US

Funding

Chitosan-plasmid DNA nanoplexes and adenoviruses as prostate cancer vaccinesR21CA133456 · NCI · UNIVERSITY OF IOWA · PI SALEM, ALIASGER K · 2008 to 2009
$338k
Engineered Optimal Adjuvant and Antigen Release from Biodegradable NanoparticlesR21CA128414 · NCI · UNIVERSITY OF IOWA · PI SALEM, ALIASGER K · 2009 to 2010
$264k
NCI NIH HHS 1R21CA128414-01A2NCI NIH HHS 1R21CA13345-01NCI NIH HHS R21 CA128414NCI NIH HHS R21 CA133456
6 · The paper itself

Abstract

Gene therapy using non-viral vectors that are safe and efficient in transfecting target cells is an effective approach to overcome the shortcomings of protein delivery of growth factors. The objective of this study was to develop and test a non-viral gene delivery system for bone regeneration utilizing a collagen scaffold to deliver polyethylenimine (PEI)-plasmid DNA (pDNA) [encoding platelet derived growth factor-B (PDGF-B)] complexes. The PEI-pPDGF-B complexes were fabricated at amine (N) to phosphate (P) ratio of 10 and characterized for size, surface charge, and in vitro cytotoxicity and transfection efficacy in human bone marrow stromal cells (BMSCs). The influence of the complex-loaded collagen scaffold on cellular attachment and recruitment was evaluated in vitro using microscopy techniques. The in vivo regenerative capacity of the gene delivery system was assessed in 5 mm diameter critical-sized calvarial defects in Fisher 344 rats. The complexes were ~100 nm in size with a positive surface charge. Complexes prepared at an N/P ratio of 10 displayed low cytotoxicity as assessed by a cell viability assay. Confocal microscopy revealed significant proliferation of BMSCs on complex-loaded collagen scaffolds compared to empty scaffolds. In vivo studies showed significantly higher new bone volume/total volume (BV/TV) % in calvarial defects treated with the complex-activated scaffolds following 4 weeks of implantation (14- and 44-fold higher) when compared to empty defects or empty scaffolds, respectively. Together, these findings suggest that non-viral PDGF-B gene-activated scaffolds are effective for bone regeneration and are an attractive gene delivery system with significant potential for clinical translation.

Indexed as

Gene Transfer TechniquesAnimalsBone RegenerationCell ProliferationCell SurvivalCollagenDNAGene ExpressionGenetic TherapyGenetic VectorsHumansMaleMesenchymal Stem CellsMicroscopy, ConfocalPlasmidsPlatelet-Derived Growth FactorCollagenDNAPlatelet-Derived Growth FactorPolyethyleneimineBone regenerationGene deliveryPlasmid DNAPlatelet derived growth factorPolyethylenimineScaffold

Identifiers

PMID24161167
PMCPMC3855224
OpenAlexW2111035980

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.