ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2013
Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 109 citations in OpenAlex.
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- A novel dual-staining method for cost-effective visualization and differentiation of microbial biofilms.Scientific reports · 2024Article
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- Coagulase-Negative Staphylococci as an Etiologic Agent of Ovine Mastitis, with a Focus on Subclinical Forms.Antibiotics (Basel, Switzerland) · 2023Review
- Characterization ofAntimicrobial agents and chemotherapy · 2023Article
- Biofilm forming multidrug resistantIranian journal of veterinary research · 2023Article
- Partnering essential oils with antibiotics: proven therapies against bovineFrontiers in cellular and infection microbiology · 2023Article
- Phenotypic and Genomic Insights into Biofilm Formation in Antibiotic-Resistant Clinical Coagulase-NegativeGenes · 2022Article
- Characterization ofBiology · 2022Article
- Incidence of biofilm formation among MRSA and MSSA clinical isolates from hospitalized patients in Israel.Journal of applied microbiology · 2022Article
- Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis.BMC veterinary research · 2022Article
- No Correlation between Biofilm-Forming Capacity and Antibiotic Resistance in EnvironmentalPathogens (Basel, Switzerland) · 2022Article
- Evaluating Tannins and Flavonoids from Traditionally Used Medicinal Plants with Biofilm Inhibitory Effects against MRGNMolecules (Basel, Switzerland) · 2022Article
- Down-regulation of biofilm-associated genes in mecA-positive methicillin-resistant S. aureus treated with M. communis extract and its antibacterial activity.AMB Express · 2021Article
- Evaluation of Virulence Determinants Using Whole-Genome Sequencing and Phenotypic Biofilm Analysis of Outbreak-LinkedFrontiers in microbiology · 2021Article
- Correlation Between Biofilm-Formation and the Antibiotic Resistant Phenotype inInfection and drug resistance · 2021Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production.
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