ArticleMethods in molecular biology (Clifton, N.J.)2014
The cyclosporin A washout assay to detect HIV-1 uncoating in infected cells.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Blockade ofmBio · 2025Article
- Cyclophilin A facilitates HIV-1 integration.Journal of virology · 2024Article
- Article
- The capsid revolution.Journal of molecular cell biology · 2024Review
- Emerging role of cyclophilin A in HIV-1 infection: from producer cell to the target cell nucleus.Journal of virology · 2023Review
- Rotten to the core: antivirals targeting the HIV-1 capsid core.Retrovirology · 2021Review
- Fullerene Derivatives Prevent Packaging of Viral Genomic RNA into HIV-1 Particles by Binding Nucleocapsid Protein.Viruses · 2021Article
- HIV-1 capsid variability: viral exploitation and evasion of capsid-binding molecules.Retrovirology · 2021Review
- Article
- Capsid Lattice Destabilization Leads to Premature Loss of the Viral Genome and Integrase Enzyme during HIV-1 Infection.Journal of virology · 2020Article
- PF74 and Its Novel Derivatives Stabilize Hexameric Lattice of HIV-1 Mature-Like Particles.Molecules (Basel, Switzerland) · 2020Article
- Characterization of HIV-1 uncoating in human microglial cell lines.Virology journal · 2020Article
- A Novel Phenotype Links HIV-1 Capsid Stability to cGAS-Mediated DNA Sensing.Journal of virology · 2019Article
- Fluorescence Microscopy Assay to Measure HIV-1 Capsid Uncoating KineticsBio-protocol · 2019Article
- Article
- Changes in HIV-1 Capsid Stability Induced by Common Cytotoxic-T-Lymphocyte-Driven Viral Sequence Mutations.Journal of virology · 2016Article
- Complex Interplay between HIV-1 Capsid and MX2-Independent Alpha Interferon-Induced Antiviral Factors.Journal of virology · 2016Article
- SUN2 Overexpression Deforms Nuclear Shape and Inhibits HIV.Journal of virology · 2016Article
- Complementary Assays Reveal a Low Level of CA Associated with Viral Complexes in the Nuclei of HIV-1-Infected Cells.Journal of virology · 2015Article
- Identification of capsid mutations that alter the rate of HIV-1 uncoating in infected cells.Journal of virology · 2015Article
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2 authors.
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Abstract
Uncoating is an early step of HIV-1 replication in which the viral capsid disassembles by p24 capsid (p24(CA)) protein dissociating from the viral complex. Although uncoating is required for HIV-1 replication, many questions remain about the mechanism of this process as well as its impact on other steps in viral replication. Here we describe a recently developed assay to study the process of uncoating in HIV-1-infected cells. The CsA washout assay is a cell-based assay that utilizes the HIV-1 restriction factor TRIM-CypA to detect and inhibit infection of coated viral complexes. Owl monkey kidney (OMK) cells are infected with a GFP reporter virus and TRIM-CypA restriction is switched on at various times postinfection allowing the kinetics of uncoating to be monitored in infected cells. This assay also can be used to examine the effect of different viral or cellular factors on the process of uncoating.
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