ArticleJournal of the mechanical behavior of biomedical materials2014
High resistance of the mechanical properties of the chondrocyte pericellular matrix to proteoglycan digestion by chondroitinase, aggrecanase, or hyaluronidase.
Article in Journal of the mechanical behavior of biomedical materials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Alteration in cartilage matrix stiffness as an indicator and modulator of osteoarthritis.Bioscience reports · 2024Pooled it
- The role of ECM mechanics in cancer mechanotransduction through unraveling the molecular machinery of integrins, FAK, and YAP signaling.Cellular & molecular biology letters · 2026Review
- Hydration as a governing state variable in the mechanical and electrical response of decellularized extracellular matrix materials.Journal of materials science. Materials in medicine · 2026Article
- Early Post‑Traumatic Osteoarthritis Features Greater Cartilage Extracellular Matrix Proteoglycan Loss Compared to the Pericellular Matrix.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- MicroRNA-mediated mechanotransduction and chondrocyte differentiation in mesenchymal stem cells.Animal cells and systems · 2026Review
- Mechanobiology of Hyaluronan: Connecting Biomechanics and Bioactivity in Musculoskeletal Tissues.Annual review of biomedical engineering · 2024Review
- Biomechanics of Chondrocytes and Chondrons in Healthy Conditions and Osteoarthritis: A Review of the Mechanical Characterisations at the Microscale.Biomedicines · 2023Review
- Superficial zone chondrocytes can get compacted under physiological loading: A multiscale finite element analysis.Acta biomaterialia · 2023Article
- Atomic Force Microscopy Micro-Indentation Methods for Determining the Elastic Modulus of Murine Articular Cartilage.Sensors (Basel, Switzerland) · 2023Article
- Hyperelastic characterization reveals proteoglycans drive the nanoscale strain-stiffening response in hyaline cartilage.Journal of biomechanics · 2023Article
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Perlecan in Pericellular Mechanosensory Cell-Matrix Communication, Extracellular Matrix Stabilisation and Mechanoregulation of Load-Bearing Connective Tissues.International journal of molecular sciences · 2021Review
- Osteoarthritis as a disease of the cartilage pericellular matrix.Matrix biology : journal of the International Society for Matrix Biology · 2018Review
- Functional properties of chondrocytes and articular cartilage using optical imaging to scanning probe microscopy.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Review
- AFM-Nanomechanical Test: An Interdisciplinary Tool That Links the Understanding of Cartilage and Meniscus Biomechanics, Osteoarthritis Degeneration, and Tissue Engineering.ACS biomaterials science & engineering · 2017Article
- Angiogenic Potential of Human Bone Marrow-Derived Mesenchymal Stem Cells in Chondrocyte Brick-Enriched Constructs Promoted Stable Regeneration of Craniofacial Cartilage.Stem cells translational medicine · 2017Article
- Osteoarthritis: toward a comprehensive understanding of pathological mechanism.Bone research · 2017Review
- Effect of hyaluronidase on tissue-engineered human septal cartilage.The Laryngoscope · 2016Article
- Mapping the Nonreciprocal Micromechanics of Individual Cells and the Surrounding Matrix Within Living Tissues.Scientific reports · 2016Article
- Pediatric laryngotracheal reconstruction with tissue-engineered cartilage in a rabbit model.The Laryngoscope · 2016Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
In articular cartilage, the extracellular matrix (ECM) and chondrocyte-associated pericellular matrix (PCM) are characterized by a high concentration of proteoglycans (PGs) and their associated glycosaminoglycans (GAGs). These molecules serve important biochemical, structural, and biomechanical roles in the tissue and differences in their regional distributions suggest that different GAG/PG species contribute to the specific biomechanical properties of the ECM and PCM. The objective of this study was to investigate region-specific contributions of aggrecan, chondroitin and dermatan sulfate, and hyaluronan to the micromechanical properties of articular cartilage PCM and ECM in situ. Cryosections of porcine cartilage underwent digestion with ADAMTS-4, chondroitinase ABC, bacterial hyaluronidase or human leukocyte elastase. Guided by immunofluorescence for type VI collagen, AFM stiffness mapping was used to evaluate the elastic properties of matched PCM and ECM regions in paired control and digested cartilage sections. These methods were used to test the hypotheses that specific enzymatic digestion of GAGs or PGs would reduce both PCM and ECM elastic moduli. Elastase, which digests a number of PGs, some types of collagen, and non-collagenous proteins, was used as a positive control. ECM elastic moduli were significantly reduced by all enzyme treatments. However, PCM micromechanical properties were unaffected by enzymatic digestion of aggrecan, chondroitin/dermatan sulfate, and hyaluronan but were significantly reduced by 24% following elastase digestion. Our results provide new evidence for high resistance of PCM micromechanical properties to PG digestion and suggest a potential role for elastase in the degradation of the ECM and PCM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.