ArticleBiomedical optics express2013
Implementation of spatial overlap modulation nonlinear optical microscopy using an electro-optic deflector.
Article in Biomedical optics express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Fabrication of a Cancer Cell Aggregate Culture Device That Facilitates Observations of Nutrient and Oxygen Gradients.Micromachines · 2024Article
- Roadmap on Label-Free Super-Resolution Imaging.Laser & photonics reviews · 2023Article
- Modulated-alignment dual-axis (MAD) confocal microscopy for deep optical sectioning in tissues.Biomedical optics express · 2014Article
- Introduction to the Novel Techniques in Microscopy feature issue.Biomedical optics express · 2013Article
- Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination.Biomedical optics express · 2013Article
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5 authors.
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Abstract
A spatial overlap modulation (SPOM) technique is a nonlinear optical microscopy technique which enhances the three-dimensional spatial resolution and rejects the out-of-focus background limiting the imaging depth inside a highly scattering sample. Here, we report on the implementation of SPOM in which beam pointing modulation is achieved by an electro-optic deflector. The modulation and demodulation frequencies are enhanced to 200 kHz and 400 kHz, respectively, resulting in a 200-fold enhancement compared with the previously reported system. The resolution enhancement and suppression of the out-of-focus background are demonstrated by sum-frequency-generation imaging of pounded granulated sugar and deep imaging of fluorescent beads in a tissue-like phantom, respectively.
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