ArticlePloS one2013
BMP12 induces tenogenic differentiation of adipose-derived stromal cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 117 citations in OpenAlex.
- Mesenchymal stem cell therapy in veterinary orthopaedics: Evidence from canine clinical medicine.Veterinary research communications · 2025Review
- Review
- Biological Augmentation Using Electrospun Constructs with Dual Growth Factor Release for Rotator Cuff Repair.ACS applied bio materials · 2025Article
- Atelocollagen exhibits superior performance compared to growth factors in upregulating proteins associated with tendon healing.PloS one · 2025Article
- Prmt7 is required for the osteogenic differentiation of mesenchymal stem cells via modulation of BMP signaling.BMB reports · 2024Article
- A Novel Bi-Directional and Bi-Temporal Delivery System for Enhancing Intrasynovial Tendon Repair.Materials and interfaces · 2024Article
- Canine Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation, Characterization, and Differentiation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Mesenchymal stem cells: An efficient cell therapy for tendon repair (Review).International journal of molecular medicine · 2023Review
- Extracellular Vesicles From Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing.Stem cells (Dayton, Ohio) · 2023Article
- Biomimetic Scaffolds for Tendon Tissue Regeneration.Biomimetics (Basel, Switzerland) · 2023Review
- Metabolic regulation of intrasynovial flexor tendon repair: The effects of dichloroacetate administration on early tendon healing in a canine model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Growth factors in the treatment of Achilles tendon injury.Frontiers in bioengineering and biotechnology · 2023Review
- Age-related cellular and microstructural changes in the rotator cuff enthesis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Optimizing Tenogenic Differentiation of Equine Adipose-Derived Mesenchymal Stem Cells (eq-ASC) Using TGFB3 Along with BMP Antagonists.Cell journal · 2022Article
- The combination of BMP12 and KY02111 enhances tendon differentiation in bone marrow-derived equine mesenchymal stromal cells (BM-eMSCs).Journal of equine science · 2022Article
- Mesenchymal Stem Cell Sheets for Engineering of the Tendon-Bone Interface.Tissue engineering. Part A · 2022Article
- Role of Yes-associated protein (YAP) in regulation of mesenchymal stem cell tenogenic differentiation.Journal of molecular histology · 2022Article
- Tendon and multiomics: advantages, advances, and opportunities.NPJ Regenerative medicine · 2021Review
- Surface-directed engineering of tissue anisotropy in microphysiological models of musculoskeletal tissue.Science advances · 2021Article
- Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cells.Stem cell research & therapy · 2021Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Adipose-derived stromal cells (ASCs) are pluripotent cells that have the capacity to differentiate into tendon fibroblasts (TFs). They are abundant in adults, easy to access, and are therefore an ideal cell source for tendon tissue engineering. Despite this potential, the molecular cues necessary for tenogenic differentiation of ASCs are unknown. Unlike other bone morphogenetic proteins (BMPs), BMP12, BMP13, and BMP14 have been reported to be less osteo-chondrogenic and to induce tendon rather than bone formation in vivo. This study investigated the effects of BMP12 and BMP14 on ASC differentiation in vitro. In canine ASCs, BMP12 effectively increased the expression of the tendon markers scleraxis and tenomodulin at both mRNA and protein levels. Consistent with these results, BMP12 induced scleraxis promoter driven-GFP and tenomodulin protein expression in mouse ASCs. Although BMP12 also enhanced the expression of the cartilage matrix gene aggrecan in ASCs, the resulting levels remained considerably lower than those detected in tendon fibroblasts. In addition, BMP12 reduced expression of the bone marker osteocalcin, but not the osteogenic transcription factor runx-2. BMP14 exhibited similar, but marginally less potent and selective effects, compared to BMP12. BMPs are known to signal through the canonical Smad pathway and the non-canonical mitogen-activated protein kinase (MAPK) pathway. BMP12 triggered robust phosphorylation of Smad1/5/8 but not Smad2/3 or p38 MAPK in ASCs. The effect was likely conveyed by type I receptors ALK2/3/6, as phosphorylation of Smad1/5/8 was blocked by the ALK2/3/6 inhibitor LDN-193189 but not by the ALK4/5/7 inhibitor SB-505124. Moreover, ALK6 was found to be the most abundant type I receptor in ASCs, with mRNA expression 100 to 10,000 times that of any other type I receptor. Collectively, results support the conclusion that BMP12 induces tenogenic differentiation of ASCs via the Smad1/5/8 pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.