ArticlePloS one2013
Proteomic analyses reveal a role of cytoplasmic droplets as an energy source during epididymal sperm maturation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 78 citations in OpenAlex.
- Regulation of mitochondrial function during spermatogenesis and sperm maturation.Cellular & molecular biology letters · 2026Review
- Biogenesis and migration of the sperm cytoplasmic droplet require ARRDC5 and TEX38.Cell reports · 2026Article
- Extracellular Vesicles, Nutrient Balance, and Redox Metabolism During Epididymal Sperm Maturation: A Perspective on the Complexity of Intercellular Interplay.Advances in anatomy, embryology, and cell biology · 2026Review
- Exploring the characters of sperm lipid peroxidation, enzymatic antioxidant activity, and spermatozoa zona binding capacity with aging in dogs.Scientific reports · 2025Article
- UBL7 is indispensable for spermiogenesis through protecting critical factors from excessive degradation by proteasomes.Nature communications · 2025Article
- TEX38 localizes ZDHHC19 to the plasma membrane and regulates sperm head morphogenesis in mice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Multi-Omics Sequencing Dissects the Atlas of Seminal Plasma Exosomes from Semen Containing Low or High Rates of Sperm with Cytoplasmic Droplets.International journal of molecular sciences · 2025Article
- Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly†.Biology of reproduction · 2024Review
- Changes in the Morphology and Antioxidant Status of European Red Deer Sperm Stored in the Epididymides and in a Liquid State.Animals : an open access journal from MDPI · 2024Article
- Spem2, a novel testis-enriched gene, is required for spermiogenesis and fertilization in mice.Cellular and molecular life sciences : CMLS · 2024Article
- The Role of Chorein Deficiency in Late Spermatogenesis.Biomedicines · 2024Article
- SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility.Nature communications · 2023Article
- Small RNA shuffling between murine sperm and their cytoplasmic droplets during epididymal maturation.Developmental cell · 2023Article
- TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The association of ODF4 with AK1 and AK2 in mice is essential for fertility through its contribution to flagellar shape.Scientific reports · 2023Article
- Investigating Visual Monitoring of the Scrotum as a Supplementary Tool for Boar Semen Quality Evaluation.Veterinary sciences · 2022Article
- Sperm RNA-mediated epigenetic inheritance in mammals: challenges and opportunities.Reproduction, fertility, and development · 2022Article
- Exposed and Sequestered Antigens in Testes and Their Protection by Regulatory T Cell-Dependent Systemic Tolerance.Frontiers in immunology · 2022Review
- Oviductal motile cilia are essential for oocyte pickup but dispensable for sperm and embryo transport.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Arrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation.Journal of extracellular vesicles · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
A small portion of cytoplasm is generally retained as the cytoplasmic droplet (CD) on the flagellum of spermatozoa after spermiation in mice. CDs are believed to play a role in osmoadaptation by allowing water entrance or exit. However, many lines of evidence suggest that CDs may have roles beyond osmoregulation. To gain more insights, we purified CDs from murine epididymal spermatozoa and conducted proteomic analyses on proteins highly enriched in CDs. Among 105 proteins identified, 71 (68%) were enzymes involved in energy metabolism. We also found that sperm mitochondria underwent a reactivation process and glycolytic enzymes were further distributed and incorporated into different regions of the flagellum during epididymal sperm maturation. Both processes appeared to require CDs. Our data suggest that the CD represents a transient organelle that serves as an energy source essential for epididymal sperm maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.