ArticlePloS one2013
In situ proximity ligation assays indicate that hemochromatosis proteins Hfe and transferrin receptor 2 (Tfr2) do not interact.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Dexamethasone induces p21FEBS open bio · 2023Article
- Distinctive modulation of hepcidin in cancer and its therapeutic relevance.Frontiers in oncology · 2023Review
- Engineering Peptide Inhibitors of the HFE-Transferrin Receptor 1 Complex.Molecules (Basel, Switzerland) · 2022Article
- Hepcidin Signaling in Health and Disease: Ironing Out the Details.Hepatology communications · 2021Review
- Appropriate Clinical Genetic Testing of Hemochromatosis Type 2-4, Including Ferroportin Disease.The application of clinical genetics · 2021Review
- Dysregulated hepcidin response to dietary iron in male mice with reduced Gnpat expression.Bioscience reports · 2020Article
- Evidence for dimerization of ferroportin in a human hepatic cell line using proximity ligation assays.Bioscience reports · 2020Article
- Haemochromatosis.Nature reviews. Disease primers · 2018Review
- The hemochromatosis protein HFE signals predominantly via the BMP type I receptor ALK3 in vivo.Communications biology · 2018Article
- HFE mRNA expression is responsive to intracellular and extracellular iron loading: short communication.Molecular biology reports · 2017Article
- Shear stress induces GαAmerican journal of physiology. Cell physiology · 2017Article
- Endofin, a novel BMP-SMAD regulator of the iron-regulatory hormone, hepcidin.Scientific reports · 2015Article
- Functional consequences of transferrin receptor-2 mutations causing hereditary hemochromatosis type 3.Molecular genetics & genomic medicine · 2015Article
- Hfe and Hjv exhibit overlapping functions for iron signaling to hepcidin.Journal of molecular medicine (Berlin, Germany) · 2015Article
- Regulation of cell surface transferrin receptor-2 by iron-dependent cleavage and release of a soluble form.Haematologica · 2015Article
- Hepcidin: regulation of the master iron regulator.Bioscience reports · 2015Review
- Relationship of Baseline Hemoglobin Level with Serum Ferritin, Postphlebotomy Hemoglobin Changes, and Phlebotomy Requirements among HFE C282Y Homozygotes.BioMed research international · 2015Article
- Article
- The erythroid function of transferrin receptor 2 revealed by Tmprss6 inactivation in different models of transferrin receptor 2 knockout mice.Haematologica · 2014Article
- The extrahepatic role of TFR2 in iron homeostasis.Frontiers in pharmacology · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hemochromatosis associated proteins HFE and Transferrin Receptor 2 (TFR2) have been shown to be important for the proper regulation of hepcidin. A number of in vitro studies using transient overexpression systems have suggested that an interaction between HFE and TFR2 is required for the regulation of hepcidin. This model of iron sensing which centers upon the requirement for an interaction between HFE and TFR2 has recently been questioned with in vivo studies in mice from our laboratory and others which suggest that Hfe and Tfr2 can regulate hepcidin independently of each other. To re-examine the postulated interaction between Hfe and Tfr2 we developed a novel expression system in which both proteins are stably co-expressed and used the proximity ligation assay to examine the interactions between Hfe, Tfr1 and Tfr2 at a cellular level. We were able to detect the previously described interaction between Hfe and Tfr1, and heterodimers between Tfr1 and Tfr2; however no interaction between Hfe and Tfr2 was observed in our system. The results from this study indicate that Hfe and Tfr2 do not interact with each other when they are stably expressed at similar levels. Furthermore, these results support in vivo studies which suggest that Hfe and Tfr2 can independently regulate hepcidin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.