ArticleScientific reports2013
Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Engineering and application of a recombinant Aspergillus niger CECT 2907 tannase to enhance the antioxidant activity of black tea.Journal of the science of food and agriculture · 2026Article
- Efficient production of L-asparaginase using recombinant Pichia pastoris and strategy to eliminate glutaminase activity.Biotechnology letters · 2026Article
- Purification-free antifungal biocontrol platform using engineered Saccharomyces cerevisiae secreting Trichoderma atroviride chitinase.Bioresources and bioprocessing · 2026Article
- Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities.Molecules (Basel, Switzerland) · 2023Review
- Optimization ofFrontiers in pharmacology · 2021Article
- A Host-Vector System for the Expression of a Thermostable Bacterial Lipase in a Locally IsolatedMicroorganisms · 2020Article
- Efficient Expression of Xylanase by Codon Optimization and Its Effects on the Growth Performance and Carcass Characteristics of Broiler.Animals : an open access journal from MDPI · 2019Article
- Integrated analysis of individual codon contribution to protein biosynthesis reveals a new approach to improving the basis of rational gene design.DNA research : an international journal for rapid publication of reports on genes and genomes · 2017Article
- A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants inFrontiers in microbiology · 2017Article
- Purification and characterization of a novel phloretin-2'-O-glycosyltransferase favoring phloridzin biosynthesis.Scientific reports · 2016Article
- Cloning, Expression, and Characterization of Siamese Crocodile (Crocodylus siamensis) Hemoglobin from Escherichia coli and Pichia pastoris.The protein journal · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mature cDNA of endochitinase from Trichoderma viride sp. was optimised based on the codon bias of Pichia pastoris GS115 and synthesised by successive PCR; the sequence was then transformed into P. pastoris GS115 via electroporation. The transformant with the fastest growth rate on YPD plates containing 4 mg/mL G418 was screened and identified. This transformant produced 23.09 U/mL of the recombinant endochitinase, a 35% increase compared to the original strain bearing the wild-type endochitinase cDNA. The recombinant endochitinase was sequentially purified by ammonia sulphate precipitation, DE-52 anion-exchange chromatography and Sephadex G-100 size-exclusion chromatography. Thin-layer chromatography indicated that the purified endochitinase could hydrolyse chito-oligomers or colloidal chitin to generate diacetyl-chitobiose (GlcNAc)₂ as the main product. This study demonstrates (1) a means for high expression of Trichoderma viride sp. endochitinase in P. pastoris using codon optimisation and (2) the preparation of chito-oligomers using endochitinase.
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