ArticlePlant physiology2013
Chloroplast phosphoglycerate kinase is involved in the targeting of Bamboo mosaic virus to chloroplasts in Nicotiana benthamiana plants.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 92 citations in OpenAlex.
- Proximity Labelling-Based Proteomics Identifies Antiviral Host Factors Associated With the Potexvirus Replicase.Molecular plant pathology · 2026Article
- Bamboo mosaic virus-induced metabolic reprogramming engages mitochondrial function to regulate redox homeostasis and defense responses in Nicotiana benthamiana.BMC plant biology · 2025Article
- The PSRP2/4 Proteins Promote Viral Infection by Interacting with the VPg Protein of TuMV.Plants (Basel, Switzerland) · 2025Article
- Role of the Transcription Factor NbTFIISL in Enhancing Bamboo Mosaic Virus Accumulation via Mitochondria Localisation.Molecular plant pathology · 2025Article
- Proviral insights of glycolytic enolase inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- The crucial role of mitochondrial/chloroplast-related genes in viral genome replication and host defense: integrative systems biology analysis in plant-virus interaction.Frontiers in microbiology · 2025Article
- Article
- Cell Fractionation and the Identification of Host Proteins Involved in Plant-Virus Interactions.Pathogens (Basel, Switzerland) · 2024Review
- Importin α2 participates in RNA interference against bamboo mosaic virus accumulation in Nicotiana benthamiana via NbAGO10a-mediated small RNA clearance.Molecular plant pathology · 2024Article
- Transcriptome analysis of genes involved in the pathogenesis mechanism of potato virus Y in potato cultivar YouJin.Frontiers in microbiology · 2024Article
- smFISH for Plants.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The Crucial Role of Chloroplast-Related Proteins in Viral Genome Replication and Host Defense against Positive-Sense Single-Stranded RNA Viruses.The plant pathology journal · 2023Review
- Cucumber STACHYOSE SYNTHASE is regulated by its cis-antisense RNA asCsSTS to balance source-sink carbon partitioning.The Plant cell · 2023Article
- The mutual interaction of glycolytic enzymes and RNA in post-transcriptional regulation.RNA (New York, N.Y.) · 2022Review
- Characterization of Virus-Inducible Orchid Argonaute 5b Promoter and Its Functional Characterization inInternational journal of molecular sciences · 2022Article
- The gibberellic acid derived from the plastidial MEP pathway is involved in the accumulation of Bamboo mosaic virus.The New phytologist · 2022Article
- Review
- The function of chloroplast ferredoxin-NADPMolecular plant pathology · 2022Article
- Photosynthesis-related genes induce resistance against soybean mosaic virus: Evidence for involvement of the RNA silencing pathway.Molecular plant pathology · 2022Article
- Voltage-dependent anion channel proteins associate with dynamic Bamboo mosaic virus-induced complexes.Plant physiology · 2022Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Bamboo mosaic virus (BaMV) is a positive-sense, single-stranded RNA virus. Previously, we identified that the chloroplast phosphoglycerate kinase (chl-PGK) from Nicotiana benthamiana is one of the viral RNA binding proteins involved in the BaMV infection cycle. Because chl-PGK is transported to the chloroplast, we hypothesized that chl-PGK might be involved in viral RNA localization in the chloroplasts. To test this hypothesis, we constructed two green fluorescent protein (GFP)-fused mislocalized PGK mutants, the transit peptide deletion mutant (NO TRANSIT PEPTIDE [NOTP]-PGK-GFP) and the nucleus location mutant (nuclear location signal [NLS]-PGK-GFP). Using confocal microscopy, we demonstrated that NOTP-PGK-GFP and NLS-PGK-GFP are localized in the cytoplasm and nucleus, respectively, in N. benthamiana plants. When NOTP-PGK-GFP and NLS-PGK-GFP are transiently expressed, we observed a reduction in BaMV coat protein accumulation to 47% and 27% that of the wild-type PGK-GFP, respectively. To localize viral RNA in infected cells, we employed the interaction of NLS-GFP-MS2 (phage MS2 coat protein) with the modified BaMV RNA containing the MS2 coat protein binding sequence. Using confocal microscopy, we observed that BaMV viral RNA localizes to chloroplasts. Furthermore, elongation factor1a fused with the transit peptide derived from chl-PGK or with a Rubisco small subunit can partially restore BaMV accumulation in NbPGK1-knockdown plants by helping BaMV target chloroplasts.
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