ArticleAmerican journal of physiology. Cell physiology2014
Rab11a-positive compartments in proximal tubule cells sort fluid-phase and membrane cargo.
Article in American journal of physiology. Cell physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- Albumin uptake and processing by the proximal tubule: physiological, pathological, and therapeutic implications.Physiological reviews · 2022Review
- Pacsin2 is required for endocytosis in the zebrafish pronephric tubule.Biology open · 2022Article
- An Adaptable Physiological Model of Endocytic Megalin Trafficking in Opossum Kidney Cells and Mouse Kidney Proximal Tubule.Function (Oxford, England) · 2022Article
- Participation of OCRL1, and APPL1, in the expression, proteolysis, phosphorylation and endosomal trafficking of megalin: Implications for Lowe Syndrome.Frontiers in cell and developmental biology · 2022Article
- Vps34/PI3KC3 deletion in kidney proximal tubules impairs apical trafficking and blocks autophagic flux, causing a Fanconi-like syndrome and renal insufficiency.Scientific reports · 2018Article
- Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway.Molecular biology of the cell · 2017Article
- Receptor-Mediated Endocytosis in the Proximal Tubule.Annual review of physiology · 2017Review
- Six2creFrs2α knockout mice are a novel model of renal cystogenesis.Scientific reports · 2016Article
- The fast-recycling receptor Megalin defines the apical recycling pathway of epithelial cells.Nature communications · 2016Article
- Flow stimulated endocytosis in the proximal tubule.Current opinion in nephrology and hypertension · 2015Review
- Shear stress-dependent regulation of apical endocytosis in renal proximal tubule cells mediated by primary cilia.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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5 authors at 1 institution in 1 country.
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Abstract
The proximal tubule (PT) reabsorbs the majority of sodium, bicarbonate, and chloride ions, phosphate, glucose, water, and plasma proteins from the glomerular filtrate. Despite the critical importance of endocytosis for PT cell (PTC) function, the organization of the endocytic pathway in these cells remains poorly understood. We have used immunofluorescence and live-cell imaging to dissect the itinerary of apically internalized fluid and membrane cargo in polarized primary cultures of PTCs isolated from mouse kidney cortex. Cells from the S1 segment could be distinguished from those from more distal PT segments by their robust uptake of albumin and comparatively low expression of γ-glutamyltranspeptidase. Rab11a in these cells is localized to variously sized spherical compartments that resemble the apical vacuoles observed by electron microscopy analysis of PTCs in vivo. These Rab11a-positive structures are highly dynamic and receive membrane and fluid-phase cargo. In contrast, fluid-phase cargoes are largely excluded from Rab11a-positive compartments in immortalized kidney cell lines. The unusual morphology and sorting capacity of Rab11a compartments in primary PTCs may reflect a unique specialization of these cells to accommodate the functional demands of handling a high endocytic load.
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